Abstract: Sensitivity of 114 Sclerotinia homoecarpa isolates to thiophanate-methyl was assayed by discriminatory concentration, and sensitivity of the tested isolates to iprodione and propiconazole was determined by mycelial growth method. 98 (85.96%) and 16 (14.04%) of the 114 isolates were sensitive and resistant to thiophanate-methy respectively, resistance was detected in 5 out of the 8 sampling locations with the history of fungicide applications. The range and mean EC50 values of S. homoeocarpa isolates were 0.012 1 to 1.264 4 and (0.525 6 ± 0.207 7) μg/mL for iprodione, 0.000 4 to 0.100 4 and (0.015 2 ± 0.020 1) for propiconazole. The highest EC50 value was 104.7 and 251.0 times greater than the lowest one for iprodione and propiconazole respectively.The results of the frequency analysis revealed that subpopulation with less sensitivity to iprodione and propiconazole had been discovered. The average EC50 values of iprodione from all the eight locations with fungicide application history showed significant difference compared to the sensitive population, while those of propiconazole from 4 locations with fungicide application history showed significant difference. Correlation analysis revealed that the 10 isolates with the least sensitivity to propiconazole were also less sensitive to iprodione, and positive correlation between iprodione and propiconazole was observed in these isolates (ρ = 0.755 4, P < 0.000 1), which indicates a possibility of multi-drug resistance in these isolates. The results will serve a useful reference for appropriate application of fungicides and effective control of dollar spot.