Abstract: A rapid, simple, sensitive and effective analytical method for the determination of flumioxazin in food (apple, grape, wheat, soybean, cabbage, and orange), soil and water based on a QuEChERS procedure was developed using high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). The samples were extracted with acetonitrile and cleaned-up by dSPE with C₁₈, GCB, PSA mixed with MgSO₄ as sorbent in different matrices. The samples were detected by using an Eclipse Plus C₁₈ column connected to an electrospray ionization source in positive mode (ESI⁺), monitoring(MRM) mode. The matrix-matched external standard calibration curves were used for quantitative analysis. The results showed that the linearity of flumioxazin in the range of 0.01-5 mg/kg, with determination coefficients higher than 0.997 6. The intra-day average recoveries ranged from 82%-104% in eight matrices at three levels (0.01, 0.1 and 1 mg/kg) and the relative standard deviation (RSD) ranged from 1.1%-7.8% (n = 5). The inter-day average recoveries ranged from 77%-109% in eight matrices at three levels (0.01, 0.1 and 1 mg/kg) and the RSD ranged from 0.1%-9.2% (n = 15). The LOQs ranged from 0.000 3-0.003 2 mg/kg, which were lower than the maximum residue limits (MRLs) of flumioxazin established by America, the European Union, Japan, and China. It can detect the residues of flumioxazin in food and environmental samples. Based on this method, the dissipation dynamics of flumioxazin in the field soil was characterized, which provided a theoretical basis for the risk assessment of food safety and environment pollution.