Abstract: In order to further explore the fungicidal activities and structure-activity relationship of cycloalkylsulfonamides, based on previous work, 11 novel 2-thiazolylamino cyclohexanesulfonamides were synthesized. Their structures were characterized by ¹H NMR, ¹³C NMR, MS and elemental analysis. The bioactivities of the target compounds was determined by adopting mycelium growth rate method, in vivo cucumber leaves method, spore germination method and in vivo tomato potted method. The bioassay exhibited that the target compounds showed good inhibitory activities against Botrytis cinerea. Among them, the inhibitory rates of compounds 7a and 7c against spore germination of B. cinerea at 10 mg/L were respectively 90% and 67% . With the application dose of 200 mg/L, the control efficacy of compound 7a against gray mold on live cucumber leaves, tomato leaves and tomato flowers were respectively 75%, 78% and 30% and the corresponding control effects of compound 7c were respectively 78%, 62%, and 44%, which were better than procymidone and worth further research.