任志杰, 毛凯凯, 李朋岳, 刘超亚, 王越, 李建洪, 万虎, 何顺. 不同温度下白背飞虱对环氧虫啶的敏感性[J]. 农药学学报, 2018, 20(4): 439-444. DOI: 10.16801/j.issn.1008-7303.2018.0056
    引用本文: 任志杰, 毛凯凯, 李朋岳, 刘超亚, 王越, 李建洪, 万虎, 何顺. 不同温度下白背飞虱对环氧虫啶的敏感性[J]. 农药学学报, 2018, 20(4): 439-444. DOI: 10.16801/j.issn.1008-7303.2018.0056
    REN Zhijie, MAO Kaikai, LI Pengyue, LIU Chaoya, WANG Yue, LI Jianhong, WAN Hu, HE Shun. Sensitivityof<italic>Sogatellafurcifera</italic>tocycloxapridatdifferenttemperatures[J]. Chinese Journal of Pesticide Science, 2018, 20(4): 439-444. DOI: 10.16801/j.issn.1008-7303.2018.0056
    Citation: REN Zhijie, MAO Kaikai, LI Pengyue, LIU Chaoya, WANG Yue, LI Jianhong, WAN Hu, HE Shun. Sensitivityof<italic>Sogatellafurcifera</italic>tocycloxapridatdifferenttemperatures[J]. Chinese Journal of Pesticide Science, 2018, 20(4): 439-444. DOI: 10.16801/j.issn.1008-7303.2018.0056

    不同温度下白背飞虱对环氧虫啶的敏感性

    Sensitivityof<italic>Sogatellafurcifera</italic>tocycloxapridatdifferenttemperatures

    • 摘要: 为明确不同温度条件下白背飞虱对环氧虫啶的敏感性,采用稻苗浸渍法测定了在18~32 ℃下环氧虫啶对白背飞虱的毒力、白背飞虱蜜露量以及酯酶 (EST)、细胞色素P450单加氧酶 (P450) 和谷胱甘肽S-转移酶 (GSTs) 3种解毒酶的比活力。结果显示:环氧虫啶对白背飞虱的毒力随温度升高而提高,32 ℃下对白背飞虱的LC50值是18 ℃下毒力的15.6倍,表明环氧虫啶对白背飞虱表现为正温度系数;温度影响白背飞虱的取食量,随温度升高,其蜜露排泄量显著增加; EST和P450比活力随温度变化而改变,其中EST比活力随温度升高呈先升高后降低的“钟形”变化趋势,P450随温度升高呈下降趋势,且处理间具有显著性差异,而GSTs随温度变化各处理间无显著差异。以上结果表明,取食量和解毒酶 (EST和P450) 可能是导致不同温度条件下白背飞虱对环氧虫啶敏感性差异的主要因素。

       

      Abstract: In order to clarify the sensitivity of Sogatella furcifera to cycloxaprid at different temperatures, the toxicity of cycloxaprid to S. furcifera at different temperatures from 18 ℃ to 32 ℃ was determined by leaf dipping method. The effects of temperatures on the secretion of honeydew and detoxification enzymes (EST, P450 and GSTs) were also detected. The results showed that cycloxaprid was a positive temperature coefficient insecticide. The toxicity of cycloxaprid against S. furcifera increased with temperature. The toxicity of cycloxaprid against S. furcifera at 32 ℃ was 15.6 times as high as that at 18 ℃. The feeding capacity and the amount of honeydew excretion increased with the increase of temperature. The activities of EST and P450 of S. furcifera also changed with temperature. With the elevation of temperature, the activity of EST increased first and then decreased. The P450 enzyme activity decreased at higher temperature. However, the activity of GSTs has no significant difference under different temperatures. The susceptibility variation of S. furcifera to cycloxaprid under different temperatures is closely related to its feeding capacity and detoxification activity (EST and P450). The above study provides a scientific reference for the rational use of cycloxaprid in the field under different environmental temperatures.

       

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