Abstract: Flucarbazone-sodium is a sulfonylurea herbicide used for controlling the weeds in wheat fields. The objective of this study is to investigate the uptake and translocation of flucarbazone-sodium and to provide evidences for formulating strategies on weed control. Uptake and translocation properties of flucarbazone-sodium was investigated through water culture method or applying flucarbazone-sodium on the leaves of Avena fatua. When A. fatua roots were incubated with flucarbazone-sodium aqueous solution at the concentration of 50 mg/L, the content percentage of flucarbazone-sodium in roots, leaf sheaths and mature leaves of A. fatua were 22%, 74% and 4% 24 h after the treatment, respectively. However, flucarbazone-sodium was not detected in apical leaves. The content percentage of flucarbazone-sodium in roots, leaf sheaths, mature leaves and apical leaves of A. fatua were 23%, 58%, 8% and 11% 48 h after the treatment, respectively. When flucarbazone-sodium was applied on the mature leaves at the concentration of 50 mg/L, the content percentage of flucarbazone-sodium in mature leaves and apical leaves of A. fatua were 57% and 43% 24 h after the treatment, respectively, while flucarbazone-sodium was not detected in roots and leaf sheaths. 48 h after the treatment, the content percentage of flucarbazone-sodium in roots, leaf sheaths, mature leaves and apical leaves of A. fatua were 1%, 1%, 68% and 30%, respectively. The results showed that flucarbazone-sodium could be absorbed by root and translocate from root to leaf and leaf sheath, meanwhile, flucarbazone-sodium could accumulate in the leaf and could be transported to the leaf sheath and root. It could be concluded that flucarbazone-sodium exhibits a unique property with two-way systemicity ability in A. fatua.