Abstract:
In order to discover novel botanical fungicides, the chemical constituents of the aerial parts of
Artemisia lavandulaefolia DC. were investigated. The aerial parts were extracted with methanol. By using liquid-liquid extraction, column chromatography, high performance preparative liquid chromatography and other techniques, 6 pure compounds were finally isolated from the ethyl acetate phase. The compounds were identified as bistetrahydrofuran lignans, including epiashchantin (
1 ), ashantin (
2 ), sesartemin (
3 ), epimagnolin A (
4 ), epiyangambin (
5 ) and diayangambin (
6 ), according to NMR and MS data. The mycelium growth rate method was used to determine the antifungal activities. The results showed that compound
1 and
3 significantly inhibited the growth of
Alternaria solani with the EC
50 value of 14.3 μg/mL and 11.0 μg/mL, respectively. Compound
3 ,
5 and
6 also showed inhibitory effect against the mycelia growth of
Rhizoctonia solani with the EC
50 value of 17.4, 42.6 and 54.3 μg/mL, respectively.