Abstract: In order to discover novel botanical fungicides, the chemical constituents of the aerial parts of Artemisia lavandulaefolia DC. were investigated. The aerial parts were extracted with methanol. By using liquid-liquid extraction, column chromatography, high performance preparative liquid chromatography and other techniques, 6 pure compounds were finally isolated from the ethyl acetate phase. The compounds were identified as bistetrahydrofuran lignans, including epiashchantin ( 1 ), ashantin ( 2 ), sesartemin ( 3 ), epimagnolin A ( 4 ), epiyangambin ( 5 ) and diayangambin ( 6 ), according to NMR and MS data. The mycelium growth rate method was used to determine the antifungal activities. The results showed that compound 1 and 3 significantly inhibited the growth of Alternaria solani with the EC₅₀ value of 14.3 μg/mL and 11.0 μg/mL, respectively. Compound 3 , 5 and 6 also showed inhibitory effect against the mycelia growth of Rhizoctonia solani with the EC₅₀ value of 17.4, 42.6 and 54.3 μg/mL, respectively.