Abstract:
For the comprehensive utilization of medicinal plant
Astragalus complanatus, the chemical constituents of its the non-meidcinal parts and their antibacterial activities were investigated preliminarily. Seven compounds were isolated by using silica gel and LH-20 gel chromatography, and their antibacterial activities against 8 species of bacteria were evaulated. After spectrometric analysis, the obtained compounds were identified as
β-sitosterol
(1) ,
β-sitostenone
(2) , lawsaritol A
(3) , 8-epidioxy-5
α,8
α-ergosta-6,22
E-dien-3
β-ol
(4) , 3′,7-dihydroxy-2′,4′-dimethoxyisoflavan
(5) ,
D-1-
O-methyl inositol
(6) and complanatuside
(7) . All of the above compounds were isolated from the aerial parts of
A. complanatus for the first time. Compound
5 showed inhibition effect on
Bacillus cereus,
Escherichia coli,
Staphylococcus aureus,
Bacillus subtilis,
Erwinia carotovora,
Pseudomonas syringae pv.
actinidiae and
Ralstonia solanacearum, and the MIC values of compound
5 against three species of plant pathogenic bacteria, including
P. syringae,
R. solanacearum and
E. carotovora, were 37.5, 18.8 and 75 μg/mL, respectively. To our best knowledge, this is the first report about the inhibition effect of
5 on plant pathogenic bacteria.