Application strategy of 25% benzobicyclon SC in controlling weedy rice
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摘要: 杂草稻Oryza sativa f. spontanea是一种危害水稻生产、具有杂草特征的水稻,筛选可防除杂草稻的除草剂可有效解决防除杂草稻的难题。为明确25%双环磺草酮悬浮剂(SC)防除杂草稻的使用策略,采用盆栽试验测定了25%双环磺草酮SC 不同用药剂量、用药时间和用药方式 (喷雾法和毒土法) 对杂草稻的防除效果及对水稻的安全性。结果表明:25%双环磺草酮SC按有效成分150~375 g/hm2的剂量分别于杂草稻萌芽期、立针期、1~2叶期和2~3叶期施用,当采用喷雾法施药时,其对杂草稻的株数防效分别达98.0%~100%、94.0%~100%、94.0%~100%和83.0%~100%,而采用毒土法施药时,其防效分别为51.0%~100%、56.0%~93.0%、27.0%~89.0%和28.0%~71.0%;在有效成分为187.5 g/hm2和375 g/hm2剂量下,喷雾法施药对水稻各项生长指标无不良影响,而毒土法施药则对水稻株高、根长、根系和植株鲜重等均有抑制作用。表明与毒土法相比,喷雾法施药具有防除效果好、使用期宽和对水稻安全性高等优点,但该药的速效性较差,施药后20~30 d方能达到最佳防效。本着高效、经济、安全的原则,推荐25%双环磺草酮SC防除杂草稻的使用策略为:于杂草稻萌芽期至1~2叶期采用喷雾法施药,推荐使用剂量为有效成分150~187.5 g/hm2,如推迟至杂草稻2~3叶期防除,则使用剂量应提高至187.5~225 g/hm2。施药时应有水层1~2 cm,施药后保持水层5 d以上。Abstract: Weedy rice (Oryza sativa f. spontanea) is a kind of rice with weed characteristics that harms rice production. Screening herbicides that can control weedy rice will effectively solve the problem of chemical control. In order to explore application strategy of 25% benzobicyclon SC, in different dosages, time and methods to control weedy rice, and safety to rice were studied by potted experiment. The results showed that 25% benzobicyclon SC had high herbicidal activity to weedy rice. Compared with the poisoned soil method, spray application had the advantages of fast acting speed,excellent control effect, wide use period and high safety to rice. After using 25% benzobicyclon SC 150-375 g a.i./hm2 on weedy rice in germination stage, needle leaf stage, 1-2 leaf stage and 2-3 leaf stage respectively, the control effect of spray method on weedy rice reached 98.0%-100%, 94.0%-100%, 94.0%-100% and 83.0%-100%, but poisoned soil method was 51.0%-100%, 56.0%-93.0%, 27.0%-89.0% and 28.0%-71.0%. Under the dosage of 187.5 g a.i./hm2 and 375 g a.i./hm2 of 25% benzobicyclon SC, spraying application had no adverse effect on growth indexes of rice, but poisoned soil method had inhibition on rice plant height, root length, root and plant fresh weight. The efficacy of the benzobicyclon appears slowly, and the best control effect is generally achieved 20-30 days after herbicide application. The optimal control period of weedy rice should be from its germination stage to 1-2 leaf stage, spraying method, and recommended dosage was 150-187.5 g a.i./hm2. If benzobicyclon SC is used during the 2-3 leaf stage of weedy rice, the dosage should be increased to 187.5 to 225 g a.i./hm2. The water layer of 1-2 cm should be established and maintained at least 5 days when benzobicyclon SC was applied.
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Key words:
- benzobicyclon /
- suspension concentrate /
- weedy rice /
- control efficacy /
- rice field /
- application strategy
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表 1 25%双环磺草酮SC在不同施药剂量及不同施药时期对杂草稻的防除效果 (药后14 d)
Table 1. Control efficacy of 25% benzobicyclon SC on weedy rice under different dosage and different application time (14 days after application)
施药方法
Application method有效成分剂量
Dosage, a.i./(g/hm2)株数防效 Plants control efficacy/% 施药时期 Application time 萌芽期
Germination stage立针期
Needle leaf stage1~2 叶期
1-2 Leaf stage2~3 叶期
2-3 Leaf stage毒土法 Poisoned soil 150.0 16.0 c 11.0 c 5.0 d 3.0 b 187.5 24.0 bc 14.0 c 7.0 cd 2.0 b 225.0 31.0 bc 18.0 c 13.0 bcd 4.0 b 375.0 42.0 b 41.0 ab 33.0 ab 5.0 b 喷雾法 Spray 150.0 32.0 bc 25.0 bc 24.0 abcd 8.0 b 187.5 46.0 b 28.0 bc 28.0 abc 21.0 ab 225.0 68.0 a 41.0 ab 33.0 ab 19.0 ab 375.0 83.0 a 49.0 a 43.0 a 30.0 a 注:同列数据后不同小写字母表示存在显著差异 (P<0.05)。Note:Different lowercases in the same column indicate significant differences (P<0.05). 表 2 25%双环磺草酮SC在不同施药剂量及不同施药时期对杂草稻的防除效果 (药后28 d)
Table 2. Control efficacy of 25% benzobicyclon SC on weedy rice under different dosage and different application time (28 days after application)
施药方法
Application
method有效成
分剂量
Dosage, a.i./(g/hm2)施药时期 Aplication time 萌芽期
Germination stage立针期
Needle leaf stage1~2 叶期
1-2 Leaf stage2~3 叶期
2-3 Leaf stage株数防效
Plants control efficacy/%鲜重防效
Fresh weight control efficacy/%株数防效
Plants control Efficacy/%鲜重防效
Fresh weight control efficacy/%株数防效
Plants control efficacy/%鲜重防效
Fresh weight control efficacy/%株数防效
Plants control efficacy/%鲜重防效
Fresh weight control efficacy/%毒土法
Poisoned soil150.0 51.0 b 52.3 b 56.0 d 8.5 b 27.0 e 2.3 c 28.0 e −0.2 c 187.5 98.0 a 77.8 a 78.0 c 14.0 b 46.0 d 6.2 c 39.0 e −4.0 c 225.0 98.0 a 92.3 a 82.0 bc 22.2 b 60.0 c 13.1 c 56.0 d −0.7 c 375.0 100.0 a 100.0 a 93.0ab 80.3a 89.0b 45.8b 71.0 c 21.3 bc 喷雾法
spray150.0 98.0 a 96.3 a 94.0 ab 88.7 a 94.0 ab 71.8 ab 83.0 bc 23.9 bc 187.5 100.0 a 100.0 a 96.0 ab 91.8 a 95.0 ab 83.2 a 92.0 ab 57.3 ab 225.0 100.0 a 100.0 a 96.0 ab 94.3 a 98.0 ab 91.4 a 95.0 ab 72.6 a 375.0 100.0 a 100.0 a 100.0 a 100.0 a 100.0 a 100.0 a 100.0 a 100.0 a 注:同列数据后不同小写字母表示存在显著差异 (P<0.05)。Note:Different lowercases in the same column indicate significant differences (P<0.05). 表 3 不同施药方法及施药剂量下25%双环磺草酮SC对水稻生长的安全性 (药后14 d)
Table 3. Effect of 25% benzobicyclon SC on rice under different application method and different dosage (14 days after application)
施药方法
Application method有效成分剂量
Dosage, a.i./(g/hm2)茎蘖数
Number of steam
and tillering株高
Plant height/cm根长
Root length/cm植株鲜重
Plant fresh weight/g根部鲜重
Root fresh weight/g毒土 Poisoned soil 187.5 2.79 a 238.54 b 57.92 b 0.64 b 0.29 c 375.0 3.06 a 253.60 b 56.69 b 0.77 b 0.39 bc 喷雾 Spray 187.5 3.50 a 320.75 a 79.79 a 1.45 a 0.66 ab 375.0 3.57 a 282.43 ab 81.54 a 1.25 a 0.49 abc CK − 3.69 a 327.01 a 90.28 a 1.71 a 0.70 a 注:同列数据后不同小写字母表示存在显著差异 (P<0.05)。Note:Different lowercases in the same column indicate significant differences (P<0.05). -
[1] DELOUCHE J C, BURGOS N R, GEALY D R, et al. Weedy rice: origin, biology, ecology and control [M]. Rome: Food and Agriculture Organization of the United Nations. 2007. [2] 王哲, 戎俊, 卢宝荣. 杂草稻的发生、危害与我国水稻生产面临的挑战[J]. 杂草科学, 2015, 33(1): 1-9.WANG Z, RONG J, LU B R. Occurrence and damage of weedy rice and its threats to rice production in China[J]. Weed Sci, 2015, 33(1): 1-9. [3] 刘睿, 强胜, 宋小玲, 等. 杂草稻苗期强竞争性的生理机制[J]. 植物保护学报, 2015, 42(1): 138-144.LIU R, QIANG S, SONG X L, et al. Physiological mechanisms of strong competition of weedy rice at seedling stage[J]. J Plant Prot, 2015, 42(1): 138-144. [4] 温广月, 沈国辉, 钱振官, 等. 杂草稻对水稻生长及产量的影响[J]. 杂草科学, 2011, 29(2): 51-53.WEN G Y, SHEN G H, QIAN Z G, et al. Effects of weedy rice(Oryza sativa f. spontanea) on the growth and yield of rice(Oryza sativa)[J]. Weed Sci, 2011, 29(2): 51-53. [5] CHAUHAN B S. Effect of weedy rice variants and weedy rice density on the growth and yield of two rice cultivars in the Philippines[J]. Canadian J Plant Pro, 2013, 1(1): 28-34. [6] SHEN X F, GAO X H, ENEJI A E, et al. Chemical control of weedy rice in precise hill-direct-seeded rice in South China[J]. Weed Biol Manag, 2013, 13(1): 39-43. doi: 10.1111/wbm.12008 [7] 余柳青, JOHNSON D E, 周勇军, 等. 杂草稻落粒粳的生物学特性与防治[J]. 植物保护学报, 2005, 32(3): 319-323. doi: 10.3321/j.issn:0577-7518.2005.03.020YU L Q, JOHNSON D E., ZHOU Y J, et al The biological characteristics and control of a weedy rice Luolijing(Oryza sativa)[J]. J Plant Prot, 2005, 32(3): 319-323. doi: 10.3321/j.issn:0577-7518.2005.03.020 [8] 张彬, 张自常, 金燕, 等. 丙草胺防治直播稻田杂草稻的技术研究[J]. 植物保护, 2015, 41(2): 205-209. doi: 10.3969/j.issn.0529-1542.2015.02.040ZHANG B, ZHANG Z C, JIN Y, et al. Control technology of pretilachlor for weedy rice in direct-seeded rice[J]. Plant Prot, 2015, 41(2): 205-209. doi: 10.3969/j.issn.0529-1542.2015.02.040 [9] 杨林, 沈浩宇, 强胜. 噁草酮防除直播稻田杂草稻的施用技术[J]. 植物保护学报, 2016, 43(6): 1033-1040.YANG L, SHEN H Y, QIANG S. A technique for oxadiazon application in controlling weedy rice in direct-seeded rice fields[J]. J Plant Prot, 2016, 43(6): 1033-1040. [10] 王芳权, 杨杰, 范方军, 等. 水稻抗咪唑啉酮类除草剂基因ALS功能标记的开发与应用[J]. 作物学报, 2018, 44(3): 324-331. doi: 10.3724/SP.J.1006.2018.00324WANG F Q, YANG J, FAN F J, et al. Development and application of the functional marker for imidazolinone herbicides resistant ALS gene in rice[J]. Acta Agron Sin, 2018, 44(3): 324-331. doi: 10.3724/SP.J.1006.2018.00324 [11] NOLDIN J A, CHANDLER J M, MCCAULEY G N, et al. Red rice (Oryza sativa) and Echinochloa spp. control in Texas Gulf coast soybean (Glycine max)[J]. Weed Technol, 1998, 12(4): 677-683. doi: 10.1017/S0890037X00044547 [12] VIDOTTO F, FERRERO A. Germination behaviour of red rice (Oryza sativa L.) seeds in field and laboratory conditions[J]. Agronomie, 2000, 20(4): 375-382. doi: 10.1051/agro:2000134 [13] FOGLIATTO S, VIDOTTO F, FERRERO A. Effects of winter flooding on weedy rice (Oryza sativa L.)[J]. Crop Prot, 2010, 29(11): 1232-1240. doi: 10.1016/j.cropro.2010.07.007 [14] CHAUHAN B S. Strategies to manage weedy rice in Asia[J]. Crop Prot, 2013, 48(2): 51-56. [15] 毕亚玲, 王曹阳, 谷刚, 等. 双环磺草酮除草活性及对水稻的安全性研究[J]. 农药学学报, 2018, 20(1): 18-24.BI Y L, WANG C Y, GU G, et al. Herbicidal activity evaluation of benzobicyclon and its safety to rice[J]. Chin J Pestic Sci, 2018, 20(1): 18-24. [16] 齐萌, 王亚楠, 康占海, 等. 25%双环磺草酮悬浮剂防除水稻移栽田杂草的效果与安全性[J]. 杂草科学, 2014, 32(1): 120-123.QI M, WANG Y N, KANG Z H, et al. Efficacy of benzobicylon and its selectivity to transplanted rice[J]. Weed Sci, 2014, 32(1): 120-123. [17] 沈国辉, 梁帝允. 中国稻田杂草识别与防除[M]. 上海: 上海科学技术出版社, 2018. [18] YOUNG M L, NORSWORTHY J K, SCOTT R C, et al. Benzobicyclon as a post-flood option for weedy rice control[J]. Weed Technol, 2018, 32(4): 371-378. doi: 10.1017/wet.2018.32 [19] 农药室内生物测定试验准则除草剂第4部分: 活性测定试验茎叶喷雾法: NY/T 1155.4—2006[S]. 2006.Pesticides guidelines for laboratory bioactivity tests. Part 4: foliar spray application test for herbicide activity: NY/T 1155.4—2006[S]. 2006 [20] 何波, 王大伟, 杨文超, 等. 对羟基苯丙酮酸双加氧酶 (HPPD) 的结构及其吡唑类除草剂的最新研究进展[J]. 有机化学, 2017, 37: 2895-2904. doi: 10.6023/cjoc201705031HE B, WANG D W, YANG W H, et al. Advances in research on 4-hydroxyphenylpyruvate dioxygenase (HPPD) structure and pyrazole-containing herbicides[J]. Chinese J Org Chem, 2017, 37: 2895-2904. doi: 10.6023/cjoc201705031 [21] MCKNIGHT B M, WEBSTER E P, BLOUIN D C. Benzobicyclon activity on common Louisiana rice weeds[J]. Weed Technol, 2018, 32: 314-318. doi: 10.1017/wet.2018.6 [22] 袁晓丹, 王亮, 王丽丽, 等. 东北地区杂草稻的抗逆性及遗传特性[J]. 延边大学农学学报, 2006, 28(4): 233-238. doi: 10.3969/j.issn.1004-7999.2006.04.002YUAN X D, WANG L, WANG L L, et al. Stress resistance and genetic characteristics of weedy rice in the Northeast area of China[J]. J Agric Sci Yanbian Univ, 2006, 28(4): 233-238. doi: 10.3969/j.issn.1004-7999.2006.04.002 [23] 董立尧, 高原, 房加鹏, 等. 我国水稻田杂草抗药性研究进展[J]. 植物保护, 2018, 44(5): 69-76.DONG L Y, GAO Y, FANG J P, et al. Research progress on the herbicide-resistance of weeds in rice fields in China[J]. Plant Prot, 2018, 44(5): 69-76. [24] 温广月, 沈国辉, 钱振官, 等. 上海地区杂草稻生物学特性初步研究[J]. 上海农业学报, 2011, 27(1): 14-18. doi: 10.3969/j.issn.1000-3924.2011.01.004WEN G Y, SHEN G H, QIAN Z G, et al. Preliminary study on biological characters of weedy rice in Shanghai[J]. Acta Agric Shanghai, 2011, 27(1): 14-18. doi: 10.3969/j.issn.1000-3924.2011.01.004 [25] 赵李霞, 叶非. HPPD抑制剂的机理与应用进展[J]. 植物保护, 2008, 34(5): 12-16. doi: 10.3969/j.issn.0529-1542.2008.05.003ZHAO L X, YE F. Mechanisms and applications of some HPPD-inhibiting herbicides[J]. Plant Prot, 2008, 34(5): 12-16. doi: 10.3969/j.issn.0529-1542.2008.05.003 -