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小白菜次生代谢物质对小菜蛾雌虫行为的调节作用

王占娣 张啦 丁百莲 史云东 王雅妮 鲁广秋 贾琳

王占娣, 张啦, 丁百莲, 史云东, 王雅妮, 鲁广秋, 贾琳. 小白菜次生代谢物质对小菜蛾雌虫行为的调节作用[J]. 农药学学报, 2021, 23(2): 323-330. doi: 10.16801/j.issn.1008-7303.2021.0016
引用本文: 王占娣, 张啦, 丁百莲, 史云东, 王雅妮, 鲁广秋, 贾琳. 小白菜次生代谢物质对小菜蛾雌虫行为的调节作用[J]. 农药学学报, 2021, 23(2): 323-330. doi: 10.16801/j.issn.1008-7303.2021.0016
Zhandi WANG, La ZHANG, Bailian DING, Yundong SHI, Yani WANG, Guangqiu LU, Lin JIA. Regulation of pakchoi's secondary metabolites on the behavior of female Plutella xylostella (Lepidoptera: Plutellidae)[J]. Chinese Journal of Pesticide Science, 2021, 23(2): 323-330. doi: 10.16801/j.issn.1008-7303.2021.0016
Citation: Zhandi WANG, La ZHANG, Bailian DING, Yundong SHI, Yani WANG, Guangqiu LU, Lin JIA. Regulation of pakchoi's secondary metabolites on the behavior of female Plutella xylostella (Lepidoptera: Plutellidae)[J]. Chinese Journal of Pesticide Science, 2021, 23(2): 323-330. doi: 10.16801/j.issn.1008-7303.2021.0016

小白菜次生代谢物质对小菜蛾雌虫行为的调节作用

doi: 10.16801/j.issn.1008-7303.2021.0016
基金项目: 云南省地方高校基础研究联合专项 (2018FH001-038);2019年云南省大学生创新创业训练计划项目"植物挥发物和信息素调节小菜蛾雌虫行为的研究" (2019B61)
详细信息
    作者简介:

    王占娣,女,讲师,研究方向为昆虫化学生态学,E-mail:zhdwang@yxnu.edu.cn

    通讯作者:

    贾琳,通信作者 (Author for correspondence),女,副教授,研究方向为分子生物学,E-mail:jl@yxnu.edu.cn

  • 中图分类号: TQ453.4;S433.4

Regulation of pakchoi's secondary metabolites on the behavior of female Plutella xylostella (Lepidoptera: Plutellidae)

  • 摘要: 为明确小白菜次生代谢物质对小菜蛾行为的调节作用,采用热回流法提取小白菜中不同极性物质,利用Y-型嗅觉仪评价这些物质对小菜蛾雌虫行为的调节效应,采用气相色谱-质谱联用仪 (GC-MS) 鉴定提取物中各化合物的化学结构。结果显示:小白菜不同极性提取物对小菜蛾雌虫行为均有一定的调节作用,其中,低极性物质在高浓度时有引诱效果,高极性物质在中、低浓度时有引诱效果。对比小白菜无水乙醇提取物和蒸馏水提取物发现,低浓度时小白菜无水乙醇提取液对小菜蛾的活性低于蒸馏水提取液。GC-MS鉴定结果显示:小白菜蒸馏水提取液中含有异柠檬酸乙酯和9,12,15-十八碳三烯醛两种化合物,其中异柠檬酸乙酯含量较高;小白菜无水乙醇提取液中含有6种化合物,含量较高的为3,7,11,15-四甲基-2-十六烯-1-醇,其次为9,12,15-十八碳三烯酸,其中,9,12,15-十八碳三烯酸和9,12,15-十八碳三烯醛具有相同的母环结构。进一步研究表明,9,12,15-十八碳三烯酸和9,12,15-十八碳三烯醛可能是小白菜调节小菜蛾行为的潜在活性物质,可用于小菜蛾绿色防治产品的开发。
  • 图  1  小白菜不同极性提取液对小菜蛾雌虫行为的调控作用

    Tr. 处理组;CK. 溶剂对照组。A. 石油醚提取液;B. 乙酸乙酯提取液;C. 二氯甲烷提取液;D. 无水乙醇提取液;E. 蒸馏水提取液。*表示处理与对照存在显著差异。

    Figure  1.  Behavioural manipulation effection of different polar extracts of pakchoi on Plutella xylostella

    Tr. Treatment; CK. Solvent control. A. Petroleum extracts; B. Dichloromethane extracts; C. Ethyl acetate extracts; D. Absolute ethyl alcohol extracts; E. Distilled water extracts. * Mean significant difference at the level of 5%.

    图  2  小白菜无水乙醇提取液与蒸馏水提取液调控小菜蛾雌虫行为比较

    Figure  2.  Comparison of the behavioural manipulation effection of extraction from pakchoi with absolute ethyl alcohol and distilled water

    图  3  小白菜无水乙醇提取液与蒸馏水提取液田间药效

    A. 蒸馏水;B. 无水乙醇;C. 蒸馏水提取液;D. 无水乙醇提取液。相同小写字母代表不同处理之间差异不显著。

    Figure  3.  Comparison of the behavioural manipulation effection of extraction from pakchoi with absolute ethyl alcohol and distilled water

    A. Distilled water; B. Absolute ethyl alcohol; C. Distilled water extracts; D. absolute ethyl alcohol extracts. The same lowercase letter are not significantly different.

    图  4  小白菜提取液全扫描总离子流色谱图 (图中数字与表1中化合物序号相同)

    A. 石油醚提取液;B. 二氯甲烷提取液;C. 乙酸乙酯提取液;D. 无水乙醇提取液;E. 蒸馏水提取液。

    Figure  4.  Total ion current chromatogram of extraction from pakchoi (The numbers in the graph showed the serial number in table 1)

    A. Petroleum ether extracts; B. Dichloromethane extracts; C. Ethylene acetate extracts; D. Absolute ethyl alcohol extracts; E. Distilled water extracts.

    表  1  小白菜不同极性溶剂提取液主要化学成分

    Table  1.   The main chemical components of extraction from pakchoi with different polar solvents

    溶剂
    Solvents
    序号
    No.
    保留时间
    Retention time/min
    反向匹配度
    R-match
    质量分数
    Mass fraction/%
    化合物
    Compound
    分子式
    Formula
    石油醚
    Petroleum ether
    1 22.670 791 0.403 2-丙基环己酮 Propyl cyclohexanone C9H14O2
    2 25.470 750 0.589 亚硫酸环己烷基己酯
    Cyclohexylmentyl hexyl ester sulfurous acid
    C14H23O3S
    3 28.500 858 2.155 6,10,14-三甲基-2-十五烷酮
    6,10,14-Trimenthyl 2-pentadecanone
    C18H36O
    4 40.112 791 1.700 邻苯二甲酸二异辛酯
    Diisooctyl phthalate
    C24H38O4
    5 41.848 864 1.383 二十五烷 n-Pentacosane C25H52
    6 43.544 845 4.357 反式-13-二十二酰胺
    trans-13-Docosenamide
    C22H43NO
    7 44.384 895 3.591 正二十七烷 n-Heptacosane C27H56
    8 46.170 741 1.051 3.6-二酮豆甾醇 Stigmastane-3,6-dione C29H48O
    9 46.784 901 5.216 正三十一烷 n-Hentriacontane C31H64
    二氯甲烷
    Dichloromethane
    1 25.466 781 4.900 烯丙基二甲基 (丙-1-炔基) 硅烷
    Allyldimethyl(prop-1-ynyl)silane
    C18H14Si
    2 28.508 929 4.120 6,10,14-三甲基-2-十五烷酮
    6,10,14-Trimethyl-2-pentadecanone,
    C18H36O
    3 38.437 860 5.180 2,2′-亚甲基-双-[6-(1,1-二甲基)-4甲基-苯酚
    2,2′-Methylenebis[6-(1,1-dimethylethyl)-4-methyl- phenol
    C22H26O2
    4 41.848 878 1.355 正三十一烷 n-Hentriacontane C31H64
    5 43.984 854 1.355 N-癸酰基吗啡啉 N-Decanoylmorpholine C14H27NO2
    6 44.399 935 3.044 正二十六烷 Hexacosane C26H54
    7 46.795 921 4.082 四十四烷 Tetratetracontane C44H88
    乙酸乙酯
    Ethylene acetate
    1 10.880 772 1.084 二羟基丙酰胺 2-Hydroxy propanamide C3H7NO2
    2 13.020 790 0.616 1,2-二戊基环丙烯 1,2-Dipentylcyclopropene C13H24
    3 19.454 831 7.536 1,2,3-丙三醇-1-乙酸酯
    1,2,3-Propanetriol-1-acetate
    C5H10O4
    4 21.415 792 2.071 1,2-甘油二乙酸酯 Glycerol 1,2-diacetate C12H23O10
    5 22.660 768 0.319 2-丙基环己酮 2-propyl-cyclohexanone C9H16O
    6 25.456 688 2.341 5,6-二 (2,2-二甲基丙基二烯)-奎酸
    5,6-Bis(2,2-dimethylpropylidene)-, (E, Z)-Decane,
    C20H38
    7 28.283 909 2.271 叶绿醇 Phytol C20H40O
    8 28.498 946 3.763 植酮 Fitone C18H36O
    9 29.198 913 1.994 1,2-苯二酸二-2-甲基丙基酯
    1,2-Benzenedicarboxylicacid, bis(2-methylpropyl) ester
    C16H22O4
    10 37.276 748 1.243 4,8,12,16-四甲基庚烷-4-内酯
    4,8,12,16-Tetramethylheptadecan-4-olide
    C21H40O2
    11 40.100 865 4.090 邻苯二甲酸十二酯
    Didodecyl Phthalate
    C32H54O4
    12 47.050 852 3.760 胆固醇杂质 Cholesta-4,6-dien-3-ol C27H44O
    无水乙醇
    Absolute ethyl alcohol
    1 33.000 780 5.440 3,7,11,15-四甲基-十六烷基-3-醇
    3,7,11,15-Tetramethyl-1-1-hexadecyn-3-ol
    C20H38O
    2 35.600 840 6.780 棕榈酸 Palmic acid C16H32O2
    3 36.100 847 1.114 棕榈酸乙酯 Ethyl palmitate C18H36O2
    4 38.350 922 13.332 3,7,11,15-四甲基-2-十六烯-1-醇
    3,7,11,15-Tetramethyl-2-hexadecen-1-ol
    C20H40O
    5 38.950 858 7.490 9,12,15-十八碳三烯酸
    9,12,15-Octadecatrienoic acid
    C18H30O2
    6 39.360 755 4.390 8,11,14-二十碳三烯酸
    8,11,14-Eicosatrienoic acid
    C20H34O2
    蒸馏水
    Distilled water
    1 35.520 674 65.730 异戊胆酸乙酯 Ethyl iso-allocholate C26H44O5
    2 39.360 849 32.870 9,12,15-十八碳三烯醛 9,12,15-Octadecatrienal C18H30O
    下载: 导出CSV
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出版历程
  • 收稿日期:  2020-06-24
  • 录用日期:  2020-10-04
  • 刊出日期:  2021-04-10

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