• 百种中国杰出学术期刊
  • 中国精品科技期刊
  • 中国高校百佳科技期刊
  • 中国高校精品科技期刊
  • 中国国际影响力优秀学术期刊
  • 中国科技核心期刊

留言板

尊敬的读者、作者、审稿人, 关于本刊的投稿、审稿、编辑和出版的任何问题, 您可以本页添加留言。我们将尽快给您答复。谢谢您的支持!

姓名
邮箱
手机号码
标题
留言内容
验证码

咪唑啉酮类除草剂的微生物降解研究进展

王新 侯佳文 柳文睿 鲍佳

王新, 侯佳文, 柳文睿, 鲍佳. 咪唑啉酮类除草剂的微生物降解研究进展[J]. 农药学学报. doi: 10.16801/j.issn.1008-7303.2021.0041
引用本文: 王新, 侯佳文, 柳文睿, 鲍佳. 咪唑啉酮类除草剂的微生物降解研究进展[J]. 农药学学报. doi: 10.16801/j.issn.1008-7303.2021.0041
Xin WANG, Jiawen HOU, Wenrui LIU, Jia BAO. Progress in microbial degradation of imidazolinone herbicides[J]. Chinese Journal of Pesticide Science. doi: 10.16801/j.issn.1008-7303.2021.0041
Citation: Xin WANG, Jiawen HOU, Wenrui LIU, Jia BAO. Progress in microbial degradation of imidazolinone herbicides[J]. Chinese Journal of Pesticide Science. doi: 10.16801/j.issn.1008-7303.2021.0041

咪唑啉酮类除草剂的微生物降解研究进展

doi: 10.16801/j.issn.1008-7303.2021.0041
基金项目: 国家自然科学基金面上基金项目 (21976124,31670515);辽宁省教育厅科学研究项目 (LJGD2020005)
详细信息
    通讯作者:

    王新,通信作者(Author for correspondence),女,副教授,主要研究方向为环境生物修复技术及污染土壤修复,E-mailwangxin110870@sut.edu.cn

  • 中图分类号: X592;S481.8;TQ450.2

Progress in microbial degradation of imidazolinone herbicides

  • 摘要: 咪唑啉酮类除草剂是中国广泛应用的除草剂,具有杀草谱广、活性高、选择性强等优点,但其在土壤中残留期长,影响后茬作物。本文综述了咪唑啉酮类除草剂甲氧咪草烟、咪唑乙烟酸和甲咪唑烟酸残留对后茬作物的影响,探究了降解咪唑啉酮类除草剂的微生物所属类群及降解途径,分析了影响微生物降解咪唑啉酮类除草剂的因素,讨论了目前除草剂污染修复存在的问题及对未来的展望。对研究咪唑啉酮类除草剂的微生物降解有一定的参考作用。
  • 1  咪唑烟酸在土壤中可能的代谢途径[34]

    1.  Possible metabolic pathways of imazapyr in soil[34]

    2  咪唑乙烟酸的降解途径[35]

    2.  Degradation pathways of imazethapyr[35]

    3  咪唑乙烟酸的降解途径[29]

    3.  Degradation pathway of imazethapyr[29]

    4  甲氧咪草烟的降解途径[28]

    4.  Degradation pathways of imazamox[28]

    表  1  咪唑乙烟酸在75 g/hm2的施用剂量下对后茬作物的影响[13]

    Table  1.   Effects of imazethapyr on succeeding crops at a dosage of 75 g/hm2[13]

    后茬作物   
    Succeeding crops  
    1 年后药害  
    Phytotoxicity after one year 
    小麦,玉米
    Wheat, corn
    正常生长
    Normal growth
    油菜
    Oilseed rape
    严重抑制,植株矮小
    Severe inhibition, plant dwarfism
    甜菜
    Beet
    严重抑制,大量死亡
    Severe suppression, mass death
    白菜
    Cabbage
    抑制,少量死亡
    Suppression, a small number of deaths
    白瓜子
    Pumpkin seed
    叶片皱缩
    Leaves shrivel
    亚麻
    Flax
    轻微抑制,叶色发黄
    Slightly suppressed, yellowish leaves
    下载: 导出CSV

    表  2  可降解咪唑啉酮类除草剂的微生物种类及特性

    Table  2.   Microbial species and characteristics of biodegradable imidazolinone herbicides

    除草剂名称及结构
    Name and structure of herbicide
    微生物    
    Microorganism    
    降解特性及
    降解率
    Degradation
    characteristic and degradation
    物化性质
    Physico-chemical property
    甲氧咪草烟
    imazamox
    鲍氏不动杆菌
    Acinetobacter baumannii IB5[28]
    37°C, pH 7.0,
    400 mg/L, 48 h:
    98.6%
    制剂为清黄色黏性液体,密度 1.39 g/cm3,蒸气压<0.013 MPa,熔点为166 °C,溶解度为:丙酮29.3 g/L
    The preparation is a clear yellow viscous liquid with a density of 1.39 g/cm3, a vapor pressure of <0.013 MPa, a melting point of 166 °C, and a solubility in acetone: 29.3 g/L
    咪唑乙烟酸
    imazethapyr
    脱色希瓦菌
    Shewanella decolorationis
    P14[29]
    30°C, pH 6.0,
    100 mg/L, 5 d:
    92.0%
    工业品为黄色到棕色结晶,有刺激性气味。纯品为白色无臭结晶体,熔点 169~173 °C,蒸气压 < 0.013 MPa(60 °C),丙酮中溶解度 (25 °C)为48.2 g/L[30],密度 1.01 g/cm3,沸点与水接近,在酸性及中性条件下稳定,有腐蚀性
    Industrial products are yellow to brown crystals with pungent odor. The pure product is white odorless crystal. melting point 169-173 °C, vapor pressure < 0.013 MPa (60 °C), solubility (25 °C) in acetone: 48.2 g/L[30], density 1.01 g/cm3, boiling point close to water, stable under acidic and neutral conditions, corrosive
    大宫链霉菌
    Streptomyces omiyaensis
    S181[22]
    30°C, pH 7.0,
    200 mg/L, 5 d:
    84.0%
    短密木霉
    Trichoderma brevicompactum[31]
    25 °C, pH 8.0,
    100 mg/kg, 7 d:
    78.4%
    表皮短杆菌
    Brevibacterium epidermidis IM9603[32]
    26.2 °C, pH 5.5,
    50 mg/L, 7 d:
    >90.0%
    粪产碱菌
    Alcaligenes faecalis
    P2[33]
    30 °C, pH 7.0,
    100 mg/kg, 2 d:
    87.8%
    曲霉属真菌黑曲霉
    Aspergillus niger
    LZ1[21]
    30 °C, pH 7.0,
    200 mg/L, 8 d:
    72.5%
    咪唑烟酸 imazapyr
    荧光假单胞菌Ⅱ型
    Pseudomonas fluorescenes
    biotype Ⅱ ZJX-5[24]
    30 °C, pH 较高,
    100 mg/L, 5 d:
    89.4%
    灰白色晶体,密度 1.34 g/cm3,熔点 169~173 °C, 沸点 425.1 °C(标况) ,折射率1.61,闪光点 210.9 °C,储存条件0~6 °C
    Off-white crystal, density: 1.34g/cm3, melting point: 169-173 °C, boiling point: 425.1 °C (standard condition), refractive index: 1.61, flash point: 210.9 °C, storage conditions: 0-6 °C
    下载: 导出CSV
  • [1] HU M F, LIU K F, QIU J S, et al. Behavior of imidazolinone herbicide enantiomers in earthworm-soil microcosms: degradation and bioaccumulation[J]. Sci Total Environ, 2020, 707: 135476. doi: 10.1016/j.scitotenv.2019.135476
    [2] 田发军, 吴艳兵, 赵欢欢, 等. 土壤微生物降解咪唑啉酮类除草剂研究进展[J]. 河南农业科学, 2014, 43(7): 1-5.

    TIAN F J, WU Y B, ZHAO H H, et al. Progress on degradation of imidazolinone herbicides by soil microorganisms[J]. J Henan Agric Sci, 2014, 43(7): 1-5.
    [3] 宋慧, 李海峰, 付楠, 等. 咪唑啉酮类除草剂及其抗性作物的发展现状[J]. 河北农业科学, 2018, 22(3): 38-41.

    SONG H, LI H F, FU N, et al. Development status of imidazolinone herbicides and their resistant crops[J]. J Hebei Agric Sci, 2018, 22(3): 38-41.
    [4] 季彬, 杜军国, 祁宏山, 等. 微生物菌剂在土壤修复中的应用[J]. 吉林农业, 2016(16): 93.

    JI B, DU J G, QI H S, et al. Application of microbial agents in soil remediation[J]. Agric Jilin, 2016(16): 93.
    [5] FALCIGLIA P P, GUIDI G D, CATALFO A, et al. Remediation of soils contaminated with PAHs and nitro-PAHs using microwave irradiation[J]. Chem Eng J, 2016, 296: 162-172. doi: 10.1016/j.cej.2016.03.099
    [6] JIA J L, WANG B B, WU Y, et al. Environmental risk controllability and management of VOCs during remediation of contaminated sites[J]. Soil Sediment Contam: Int J, 2016, 25(1): 13-25. doi: 10.1080/15320383.2016.1085834
    [7] CYCOŃ M, MROZIK A, PIOTROWSKA-SEGET Z. Bioaugmentation as a strategy for the remediation of pesticide-polluted soil: a review[J]. Chemosphere, 2017, 172: 52-71. doi: 10.1016/j.chemosphere.2016.12.129
    [8] SILVA V P, MOREIRA-SANTOS M, MATEUS C, et al. Evaluation of Arthrobacter aurescens strain TC1 as bioaugmentation bacterium in soils contaminated with the herbicidal substance terbuthylazine[J]. PLoS One, 2015, 10(12): e0144978. doi: 10.1371/journal.pone.0144978
    [9] HUANG X, HE J, YAN X, et al. Microbial catabolism of chemical herbicides: microbial resources, metabolic pathways and catabolic genes[J]. Pestic Biochem Physiol, 2017, 143: 272-297. doi: 10.1016/j.pestbp.2016.11.010
    [10] 丁伟, 白鹤, 程茁, 等. 咪唑乙烟酸降解菌的分离、鉴定及其降解特性研究[J]. 环境科学, 2008, 29(5): 1359-1362.

    DING W, BAI H, CHENG Z, et al. Isolation, identification and degradation characteristics of imazethapyr degrading bacteria[J]. Chin J Environ Sci, 2008, 29(5): 1359-1362.
    [11] 张可鑫. 5种大豆田除草剂残留的垂直分布及对后茬作物生长的影响[D]. 大庆: 黑龙江八一农垦大学, 2020.

    ZHANG K X. Vertical distribution of herbicide residues in 5 soybean fields and their effects on growth of succeeding crops[D]. Daqing: Heilongjiang Bayi Agricultural Reclamation University, 2020.
    [12] 刘延, 刘波, 王险峰, 等. 中国化学除草问题与对策[J]. 农药, 2005, 44(7): 289-293.

    LIU Y, LIU B, WANG X F, et al. Problems and solutions for chemical weed control in China[J]. Pesticides, 2005, 44(7): 289-293.
    [13] 黄春艳, 陈铁保, 王宇, 等. 咪唑啉酮类除草剂对后茬作物安全性研究初报[J]. 农药学学报, 2001, 3(2): 29-34.

    HUANG C Y, CHEN T B, WANG Y, et al. Study on safety of imidazolinone herbicides to succeeding crops[J]. Chin J Pestic Sci, 2001, 3(2): 29-34.
    [14] 史伟. 咪唑乙烟酸对土壤微生物、酶活性的影响及其降解真菌的筛选[D]. 北京: 中国农业科学院, 2010.

    SHI W. Effects of imazethapyr on soil microorganism, enzyme activity and isolation of imazethapyr degrading fungi[D]. Beijing: Chinese Academy of Agricultural Science, 2010.
    [15] 李新安, 李广领, 谢兰芬, 等. 咪唑乙烟酸残留对后茬作物油菜生理指标的影响[J]. 农药, 2015, 54(9): 671-673.

    LI X A, LI G L, XIE L F, et al. Effects of imazethapyr residue on physiological indices of succeeding crop of rapeseed[J]. Agrochemicals, 2015, 54(9): 671-673.
    [16] 谷珊山. 两种长残效除草剂对作物的影响及药害修复[D]. 新乡: 河南科技学院, 2015.

    GU S S. The effects of two herbicides on crop and remediation of biochar[D]. Xinxiang: Henan Institute of Science and Technology, 2015.
    [17] 苏旺苍, 孙兰兰, 吴仁海, 等. 甲咪唑烟酸模拟残留对后茬作物的敏感性[J]. 农药, 2014, 53(4): 260-262.

    SU W C, SUN L L, WU R H, et al. Sensitivity of succeeding crops to simulated imazapic residue in laboratory[J]. Pesticides, 2014, 53(4): 260-262.
    [18] 苏旺苍, 孙兰兰, 张强, 等. 甲咪唑烟酸在土壤中的残留对后茬小麦幼苗生长和光合作用的影响[J]. 麦类作物学报, 2013, 33(6): 168-173.

    SU W C, SUN L L, ZHANG Q, et al. Effects of imazapic residues on the growth and photosynthetic parameters of wheat seedlings as succeeding crop[J]. J Triticeae Crop, 2013, 33(6): 168-173.
    [19] 苏旺苍. 甲咪唑烟酸残留对后茬作物的影响及土壤修复研究[C]//第十一届全国杂草科学大会论文集. 长沙, 2013: 101.

    SU W C. Effects of imazapic residue on succeeding crops and soil remediation[C]//The 11th National Weed Science Congress. Changsha, 2013: 101.
    [20] 刘亚光, 庞福德. 咪唑乙烟酸高效降解菌降解性能的研究[J]. 植物保护, 2009, 35(2): 63-66.

    LIU Y G, PANG F D. Degradation characteristics of the highly efficient degradative bacteria of imazethapyr[J]. Plant Prot, 2009, 35(2): 63-66.
    [21] 曹知平, 许景钢, 李淑芹, 等. 黑曲霉LZ1降解咪唑乙烟酸的特性[J]. 东北农业大学学报, 2010, 41(7): 66-70.

    CAO Z P, XU J G, LI S Q, et al. Degradation of imazethapyr by Aspergillus sp. LZ1[J]. J Northeast Agric Univ, 2010, 41(7): 66-70.
    [22] 霍莹, 许景钢, 李淑芹, 等. 咪唑乙烟酸降解菌S181的分离鉴定及其降解特性[J]. 环境科学, 2011, 32(5): 1518-1523.

    HUO Y, XU J G, LI S Q, et al. Isolation and identification of imazethapyr degradable actinomyces S181 and its degradation characteristics[J]. Environ Sci, 2011, 32(5): 1518-1523.
    [23] 臧海莲, 熊明华, 成小松, 等. 一株咪唑乙烟酸降解菌的分离鉴定及其降解特性[J]. 作物杂志, 2010(3): 40-44.

    ZANG H L, XIONG M H, CHENG X S, et al. Isolation and characteristics of a imazethapyr degradation bacterium[J]. Crops, 2010(3): 40-44.
    [24] 王学东, 欧晓明, 王慧利, 等. 除草剂咪唑烟酸在土壤中的微生物降解研究[J]. 土壤学报, 2004, 41(1): 156-159.

    WANG X D, OU X M, WANG H L, et al. Study on degradation of imzapyr by microorganism in soil[J]. Acta Pedol Sin, 2004, 41(1): 156-159.
    [25] 王学东, 欧晓明, 王慧利, 等. 除草剂咪唑烟酸高效降解菌的筛选及其降解性能的研究[J]. 农业环境科学学报, 2003, 22(1): 102-105.

    WANG X D, OU X M, WANG H L, et al. Optimized cultivation of highly-efficient degradation bacterial strains and their degradation ability towards imazapyr[J]. J Agro - Environ Sci, 2003, 22(1): 102-105.
    [26] 王学东, 欧晓明, 王慧利, 等. 咪唑烟酸高效降解菌的降解特性[J]. 中国环境科学, 2003, 23(1): 21-24.

    WANG X D, OU X M, WANG H L, et al. The degradation characteristics of highly efficient degradation bacteria of imazapyr[J]. China Environ Sci, 2003, 23(1): 21-24.
    [27] 杨鑫. 咪唑啉酮类除草剂降解菌的分离鉴定及降解特性研究[D]. 哈尔滨: 黑龙江大学, 2011.

    YANG X. Isolation, identification and degradation characteristics of imidazolinone herbicides degradation bacteria[D]. Harbin: Heilongjiang University, 2011.
    [28] LIU C G, YANG X, LAI Y, et al. Imazamox microbial degradation by common clinical bacteria: Acinetobacter baumannii IB5 isolated from black soil in China shows high potency[J]. J Integr Agric, 2016, 15(8): 1798-1807. doi: 10.1016/S2095-3119(16)61344-8
    [29] 陈玉洁. 咪唑乙烟酸降解菌分离、鉴定及降解特性研究[D]. 长春: 吉林农业大学, 2011.

    CHEN Y J. Isolation, identification and characterization of an imazethapyr-degrading strain[D]. Changchun: Jilin Agricultural University, 2011.
    [30] 刘长令. 世界农药大全: 除草剂卷[M]. 北京: 化学工业出版社, 2002.

    LIU C L. World pesticide encyclopedia: herbicide volume[M]. Beijing: Chemical Industry Press, 2002.
    [31] 刘晴, 刘宇彤, 邓世林, 等. 短密木霉降解咪唑乙烟酸候选基因的筛选[J]. 华中农业大学学报, 2019, 38(4): 63-70.

    LIU Q, LIU Y T, DENG S L, et al. Screening candidate genes of degradating imazethapyr by Trichoderma brevicompactum[J]. J Huazhong Agric Univ, 2019, 38(4): 63-70.
    [32] 吕翻洋, 许泽华, 毛晓洁, 等. 咪唑乙烟酸降解菌的分离鉴定及降解条件优化[J]. 生物技术通报, 2017, 33(10): 163-168.

    LÜ F Y, XU Z H, MAO X J, et al. Isolation and identification of an imazethapyr-degrading bacterium, and optimization of biodegradation conditions[J]. Biotechnol Bull, 2017, 33(10): 163-168.
    [33] 李志国. 降解菌、大豆和咪唑乙烟酸对土壤微生物多样性的影响[D]. 哈尔滨: 东北林业大学, 2018.

    LI Z G. Effects of highly efficient degrading microbe, soybean and imazethapyr on soil microbial diversity[D]. Harbin: Northeast Forestry University, 2018.
    [34] 王学东, 王慧利, 樊德方. 咪唑烟酸在土壤中的微生物降解及其代谢物分析[J]. 环境科学研究, 2004, 17(6): 42-45, 53.

    WANG X D, WANG H L, FAN D F. Degradation and metabolism of imazapyr by microorganisms in soils[J]. Res Environ Sci, 2004, 17(6): 42-45, 53.
    [35] 金雷. 咪草烟降解菌株QC-13的分离鉴定、降解特性及应用研究[D]. 南京: 南京农业大学, 2012.

    JIN L. Isolation, identification, degradating characteristics and soil bioremediation application of imazethapyr-degrading strain QC-13[D]. Nanjing: Nanjing Agricultural University, 2012.
    [36] 卢美名, 尹雯悦, 刘传龙, 等. 除草剂微生物降解的研究进展[J]. 湖北农业科学, 2019, 58(3): 5-8.

    LU M M, YIN W Y, LIU C L, et al. Research progress on biodegradation of herbicides[J]. Hubei Agric Sci, 2019, 58(3): 5-8.
    [37] WANG X D, LIU X J, WANG H L, et al. Utilization and degradation of imazaquin by a naturally occurring isolate of Arthrobacter crystallopoietes[J]. Chemosphere, 2007, 67(11): 2156-2162.
    [38] 倪子钧. 烟嘧磺隆和甲氧咪草烟优势菌的筛选及其降解特性研究[D]. 沈阳: 沈阳工业大学, 2020.

    NI Z J. Screening and degradation characteristics study of nicosulfuron and imazamox degrading bacteria[D]. Shenyang: Shenyang University of Technology, 2020.
    [39] RECK L, REIMCHE G B, ALVES C R, et al. Efeito dos herbicidas imazapire imazapiquena comunidade fitoplanctônica em lavoura de arroz irrigado[J]. Iheringia Ser Bot, 2018, 73(2): 298-307.
    [40] 孙诗雨. 复合菌系对磺酰脲类除草剂降解性能的研究[D]. 沈阳: 沈阳工业大学, 2019.

    SUN S Y. Study on degradation property of sulfonylurea herbicides by composite bacteria[D]. Shenyang: Shenyang University of Technology, 2019.
    [41] ZHANG C P, LIU X G, DONG F S, et al. The effect of imazethapyr on soil microbes in soybean fields in northeast China[J]. Chem Ecol, 2010, 26(3): 173-182.
    [42] 陈旭艳, 葛宝坤, 常春艳. 咪唑啉酮类除草剂环境行为研究进展[J]. 精细化工中间体, 2010, 40(2): 1-6.

    CHEN X Y, GE B K, CHANG C Y. Advances in studies on the environmental behaviors of imidazolinone herbicides[J]. Fine Chem Intermed, 2010, 40(2): 1-6.
    [43] 张悦周, 吴耀国, 胡思海, 等. 微生物降解有机磷农药的研究进展[J]. 化工环保, 2007, 27(6): 514-519.

    ZHANG Y Z, WU Y G, HU S H, et al. Research advances in biodegradation of organic phosphorus pesticides[J]. Environ Prot Chem Ind, 2007, 27(6): 514-519.
  • 加载中
计量
  • 文章访问数:  402
  • HTML全文浏览量:  202
  • 被引次数: 0
出版历程
  • 收稿日期:  2020-06-22
  • 录用日期:  2020-10-22
  • 网络出版日期:  2020-11-30

目录

    /

    返回文章
    返回