Abstract: The application methods and application efficacy of pesticide are closely related to their uptake and translocation behaviors in the plants. The uptake and translocation behaviors of pesticides on the surface and inside of plants are not only related to the physical and chemical properties of pesticides, but also affected by some factors, such as plants species, growth period, growth conditions and application technologies. The uptake and translocation of pesticides in plants and the influencing factors have important guiding significance for the selection of application technologies and the improvement effective utilization of pesticides. This review summarized the uptake and translocation behaviors of pesticides in the plants, the relationship between conduction modes and application technologies of pesticides, and the factors influencing the uptake and translocation behaviors of pesticides. The measures to improve the uptake and translocation behaviors of pesticides were also proposed, which will provide some theoretical support for the safe and reasonable application of pesticides.