Abstract: To investigate the dissipation and residues of ningnanmycin in ginseng, field trials of ningnanmycin residues in ginseng and its plants were conducted in Baishan City, Fusong County, Yanji City of Jilin Province, and Huanren County of Liaoning Province in 2019. The samples were extracted by 0.2% formic acid aqueous solution, cleaned up with PLS+PXC solid phase extraction column, separated by XSelect® HSS T3 column, and determined using HPLC-MS/MS. At 0.1, 0.2 and 1 mg/kg spiking levels, the average recoveries of ningnanmycin in fresh ginseng, ginseng plants were 90%-99%, 92%-94%, with the relative standard deviations (RSD) were 4.7%-5.6%, 4.0%-5.6%, respectively. At 0.2, 1 and 2 mg/kg spiking levels, the average recoveries of ningnanmycin in dried ginseng was 88%-95% with the RSD was 1.4%-10%. The limit of quantification (LOQ) of fresh ginseng and ginseng plants were both 0.1 mg/kg, and that of dried ginseng was 0.2 mg/kg. The results of the dissipation dynamic test in Baishan and Huanren showed that the half-lives of ningnanmycin on ginseng plants were 11.77 d and 0.76 d. The terminal residue results in 4 test places showed that the terminal residues of ningnanmycin on fresh ginseng, ginseng plants, and dried ginseng were <LOQ, <LOQ-0.152 mg/kg, and <LOQ, respectively. The results indicated that the harvested ginseng with the pre-harvest interval of 14 days would be safe for consumption after spraying 8% ningnanmycin AS at the recommended dosage of 675-900 g/hm² (54-72 g a.i./hm²) 3 times to prevent and treat ginseng disease.