Abstract: To clarify the effect of nine commonly used pesticides, including tebuconazole, prochloraz, prothioconazole, difenoconazole, thifluzamide, azoxystrobin, chlorantraniliprole, indoxacarb and emamectin benzoate against natural enemy insect Trichogramma chilonis, the residual film method was used to determine the toxicities of nine pesticides and assess the effect of mixtures between different kinds of fungicides on the survival of T. chilonis. The results showed that prothioconazole, difenoconazole, thifenamide and azoxystrobin had low-risk against T. chilonis indicating by the median lethal rate (LR₅₀) > 1 832 g/hm² and the half lethal time (LT₅₀) over 4 d. Indoxacarb and chlorantraniliprole were also low-risk agents, and both with LR₅₀ > 916 g/hm². The LT₅₀ values of them were 2.94 d and 0.86 d, respectively. The LR₅₀ of prochloraz and tebuconazole were 106 g/hm² and 207 g/hm², respectively, and the LT₅₀ were 0.27 d and 0.35 d, respectively, which indicated them to be medium-risk agents. Emamectin benzoate was a high-risk agent with LR₅₀ and LT₅₀ of 0.53 g/hm² and 0.16 d, respectively. When prochloraz or tebuconazole was mixed respectively with prothioconazole and thifluzamide, and tebuconazole mixed with azoxystrobin, significant reduction in the mortality rate of T. chilonis in the mixed treatment group was observed, and no significant difference with that of the acetone blank control. The results showed that chlorantraniliprole and indoxacarb had low toxicity to T. chilonis, and the usage of the mixture of different fungicides could reduce the toxicity of single dose to a certain extent. This study has provided theoretical instruction for the rational use of insecticides and fungicides in wasp release period.