Abstract: A high performance anion exchange-integral pulse method was established to determine the content of free glucosamine in amino-oligosaccharin technical concentrates and aqueous solution. Using the DionexAminoTrap Column and DionexCarboPac PA100 anion exchange column as the separation column, the target free glucosamine was eluted with 17 mmol/L sodium hydroxide, and the chitosan oligosaccharide was flushed by 200 mmol/L sodium hydroxide. The flow rate was 1.0 mL/min, and the total analysis time was 30 min. Under the optimal separation conditions, the interference of chitosan oligosaccharides on glucosamine could be eliminated. The detection limits of glucosamine reached 0.01 mg/L (S/N=3), the calibration curve ranged from 0.02 to 10 mg/L, and the correlation coefficients was 0.999 9. For the analysis of commercially available amino-oligosaccharin technical concentrates and aqueous solution at 3 replicates, the relative standard deviation was less than 1.9%, and the spiked recoveries were between 90%-99%, which indicated that the established method was accurate and reliable. Therefore, this method could be used for the detection of free glucosamine in amino-oligosaccharin technical concentrates and aqueous solution, and could provide method for the quality evaluation of the amino-oligosaccharin pesticides.