嘧菌酯/聚丙烯腈微球的制备及缓释性能

张钟凯; 应彩娇; 程志强; 于晓斌

doi:10.16801/j.issn.1008-7303.2022.0014

嘧菌酯/聚丙烯腈微球的制备及缓释性能

doi: 10.16801/j.issn.1008-7303.2022.0014

1.
吉林农业大学植物保护学院，长春 130118
2.
吉林农业大学资源与环境学院，长春 130118

基金项目: 吉林省科技厅计划项目(20210509025RQ)；吉林省发展改革委员会能力建设项目(2019C050-9).

详细信息

作者简介:
张钟凯，546895471@qq.com

应彩娇，1315340296@qq.com

通讯作者:
于晓斌，446745723@qq.com

中图分类号: S481.9；TQ450.6
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- 文章访问数: 305
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- 被引次数: 0
出版历程
- 收稿日期: 2021-05-19
- 录用日期: 2021-12-21
- 网络出版日期: 2022-02-18
- 刊出日期: 2022-06-10

Preparation and release properties of azoxystrobin/ polyacrylonitrile microspheres

1.
College of Plant Protection, Jilin Agricultural University, Changchun 130118, China
2.
College of Resources and Environment, Jilin Agricultural University, Changchun 130118, China

Funds: the Jilin Province Science and Technology Development Project (20210509025RQ), Jilin Province Innovation Capacity Building Fund Project (2019C050-9).

摘要

摘要: 采用聚丙烯腈(PAN)为原料，通过静电喷雾技术制备了载有嘧菌酯的PAN微球。研究了工作电压、嘧菌酯与PAN质量比对微球形貌与性能的影响，并通过释放试验评价了载药微球的缓释性能。结果表明：静电纺丝机的工作电压是控制微球粒径大小的主要因素，其粒径随电压增大而减小，最小可至0.86 μm，而嘧菌酯与PAN的质量比则主要影响微球的载药量与包封率，微球载药量最高可达34.53%，包封率最高可达79.78%。此外，载药微球在3种不同pH的缓冲溶液中均实现了长达400 min的稳定释放，表明其具有出色的缓释性能。在静电纺丝机工作电压15 kV、工作距离20 cm、PAN溶液质量浓度2%、m(嘧菌酯) : m(PAN) = 1 : 2 及进样速率1.25 mL/h的条件下，可制备具有良好形貌与缓释性能的嘧菌酯/PAN微球，本研究可为农药减量增效提供一种技术路径。
- 嘧菌酯 /
- 聚丙烯腈 /
- 静电喷雾 /
- 微球 /
- 缓释性能
Abstract: In this study, polyacrylonitrile (PAN) was used to prepare PAN microspheres loaded with azoxystrobin by electrostatic spraying technology. The effects of working voltage and mass ratio of azoxystrobin and PAN on the morphology and properties of microspheres were investigated. The sustained-release properties of the drug-loaded microspheres were evaluated by release tests. The results show that the working voltage of the electrospinning machine is the main factor controlling the particle size of the microspheres. The particle size decreases with the increase of the voltage and the minimum size can reach 0.86 μm. While the mass ratio of azoxystrobin and PAN mainly affected the loading capacity and encapsulation efficiency of the microspheres. The loading capacity of the microspheres can reach up to 34.53%, and the encapsulation efficiency can reach up to 79.78%. In addition, the drug-loaded microsphere achieved stable release for up to 400 min in three buffer solutions with different pH, indicating its excellent sustained-release performance. Collectively, under the condition that the working voltage of the electrospinning machine is 15 kV, the working distance is 20 cm, the mass concentration of PAN solution is 2%, m(azoxystrobin) : m (PAN) = 1 : 2 and the injection speed is 1.25 mL/h, the prepared azoxystrobin/PAN microspheres exhibited good morphology and sustained-release properties. This study provides a technical approach for pesticide reduction and efficiency enhancement.
- azoxystrobin /
- polyacrylonitrile /
- electrostatic spray /
- microsphere /
- sustained release properties

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图 1 静电喷雾实验装置图

Figure 1. Diagram of electrostatic spray experimental device

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图 2 工作电压分别在13、14 和15 kV下载药微球SEM图(A~C)和粒径分布(a~c)

Figure 2. The SEM images(A-C) and particle size distribution(a-c) of the microspheres recorded under the working voltages of 13, 14 and 15 kV

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图 3 不同pH缓冲溶液中的累积释药曲线

(a)载药微球中的嘧菌酯； (b)嘧菌酯原药。

Figure 3. Cumulative release curve in buffer solutions with different pH

　　　(a) azoxystrobin in polyacrylonitrile microspheres; 　　　　　　　　　　(b) azoxystrobin.

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表 1 不同pH值磷酸盐缓冲溶液的配制

Table 1. Preparation of phosphate buffer solutions with different pH

pH	体积分数 Volume ratio, V/%
pH	0.2 mol/L KH₂PO₄	0.2 mol/L K₂HPO₄	0.1 mol/L TC
5	−	51.00	49.00
7	40.00	60.00	−
8	5.50	94.50	−

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表 2 嘧菌酯与PAN质量比对载药微球的影响

Table 2. Influence of the mass ratio of azoxystrobin and polyacrylonitrile on drug-loaded microspheres

m (嘧菌酯) : m (PAN)m (azoxystrobin) : m (PAN)	直径Diameter/ µm	载药量Loading capacity/%	包封率Encapsulation efficiency/%
1 : 2	0.89 ± 0.09	26.59 ± 0.36	79.78
1 : 1	0.87 ± 0.07	30.55 ± 1.34	61.10
2 : 1	0.86 ± 0.08	34.53 ± 1.31	51.79

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表 3 不同剂型嘧菌酯对黄瓜炭疽病的防治效果

Table 3. Control efficacy of azoxystrobin with different formulations on cucumber anthracnose

处理 Treatment	有效成分质量浓度Concentration, a.i/(g/L)	防治效果 Control efficacy/%
处理 Treatment	有效成分质量浓度Concentration, a.i/(g/L)	处理后7 d7 d after spray	处理后14 d14 d after spray
清水 CK (Water)	−	−	−
25%嘧菌酯悬浮剂 azoxystrobin 25% SC	0.2	79.58 Bb	54.46 Bb
嘧菌酯/PAN微球悬浮剂 azoxystrobin/PAN microspheres SC	0.2	51.19 Aa	26.36 Aa
嘧菌酯/PAN微球悬浮剂 azoxystrobin/PAN microspheres SC	0.3	71.12 Bb	55.41 Bb
	0.4	73.48 Bb	61.08 Bb
注：表中数据后的大小写字母分别表示在5%、1%水平下的显著性差异。Note: The upper and lower case letters after the data in the table indicate significant differences at the 5% and 1% levels, respectively.

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