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深绿木霉T2菌株发酵液蛋白提取物TraT2A抑菌条件优化及其对百合叶斑病的田间防治效果

陈应娥 梁巧兰 魏列新 同发宇 王冬

陈应娥, 梁巧兰, 魏列新, 同发宇, 王冬. 深绿木霉T2菌株发酵液蛋白提取物TraT2A抑菌条件优化及其对百合叶斑病的田间防治效果[J]. 农药学学报, 2022, 24(4): 782-788. doi: 10.16801/j.issn.1008-7303.2022.0031
引用本文: 陈应娥, 梁巧兰, 魏列新, 同发宇, 王冬. 深绿木霉T2菌株发酵液蛋白提取物TraT2A抑菌条件优化及其对百合叶斑病的田间防治效果[J]. 农药学学报, 2022, 24(4): 782-788. doi: 10.16801/j.issn.1008-7303.2022.0031
CHEN Ying’e, LIANG Qiaolan, WEI Liexin, TONG Fayu, WANG Dong. Optimization of inhibition conditions of Trichoderma atroviride T2 fermentation liquid protein extract TraT2A and its field control efficacy for lily leaf spot disease[J]. Chinese Journal of Pesticide Science, 2022, 24(4): 782-788. doi: 10.16801/j.issn.1008-7303.2022.0031
Citation: CHEN Ying’e, LIANG Qiaolan, WEI Liexin, TONG Fayu, WANG Dong. Optimization of inhibition conditions of Trichoderma atroviride T2 fermentation liquid protein extract TraT2A and its field control efficacy for lily leaf spot disease[J]. Chinese Journal of Pesticide Science, 2022, 24(4): 782-788. doi: 10.16801/j.issn.1008-7303.2022.0031

深绿木霉T2菌株发酵液蛋白提取物TraT2A抑菌条件优化及其对百合叶斑病的田间防治效果

doi: 10.16801/j.issn.1008-7303.2022.0031
基金项目: 甘肃省农牧厅项目 (GNSW-2016-25);兰州市科技局项目 (2018-4-78);甘肃省科技重大专项 (18ZD2NA010);甘肃农业大学学科建设基金 (GAU-XKJS-2018-155).
详细信息
    作者简介:

    陈应娥, 1470857734@qq.com

    通讯作者:

    梁巧兰,liangql@gsau.edu.cn

  • 中图分类号: S482.2;TQ450.2

Optimization of inhibition conditions of Trichoderma atroviride T2 fermentation liquid protein extract TraT2A and its field control efficacy for lily leaf spot disease

Funds: Agriculture and Animal Husbandry Program of Gansu Province (GNSW-2016-25); Science and Technology Bureau Program of Lanzhou (2018-4-78); The Major Science and Technology Program of Gansu Province (18ZD2NA010); Discipline Construction Fund of Gansu Agricultural University (GAU-XKJS-2018-155).
  • 摘要: 为了提高深绿木霉Trichoderma atroviride T2菌株发酵液蛋白提取物TraT2A的抑菌活性和光稳定性,采用菌丝生长速率法,通过对紫外保护剂 (抗坏血酸、腐殖酸) 和辅助剂 (IE-08、SP-4821A和SP-4821B) 的筛选,优化了TraT2A对百合叶斑病菌Alternaria alternata的抑菌条件,并测定了其对百合叶斑病的田间防治效果。结果表明:TraT2A在200.00 mg/mL下对百合叶斑病菌A.alternata的抑菌作用较好,抑制率为72.12%;随着紫外光照时间的延长,TraT2A的抑菌活性逐渐降低,添加5.00 mg/mL的腐殖酸对TraT2A有较好的紫外保护作用,抑制率 (69.01%) 比不加紫外保护剂对照 (29.37%) 提高39.64%;添加0.17 mg/mL 的辅助剂SP-4821A对TraT2A活性的提高效果最好,抑制率 (95.13%) 比TraT2A对照 (69.16%) 提高25.97%。按照优化后的配方:200 g TraT2A (质量浓度为200.00 mg/mL) + 5 g腐殖酸 (质量浓度为5.00 mg/mL) + 0.17 g SP-4821A (质量浓度为0.17 mg/mL) + 794.83 mL灭菌水,进行田间药效试验,结果发现其对百合叶斑病的防治效果为80.37%。该研究结果为进一步开发防治百合病害的TraT2A制剂提供了理论依据。
  • 表  1  TraT2A对百合叶斑病菌的抑菌作用

    Table  1.   The inhibition effect of TraT2A on A. alternata

    TraT2A 质量浓度
    TraT2A concentration/(mg/mL)
    抑制率
    Inhibition rate/%
    200.0072.12 ± 0.25 aA
    100.0067.31 ± 0.68 bB
    66.6726.92 ± 0.57 cC
    注:表中数据为平均值 ± 标准误差。同列数据后不同小写和大写字母分别表示经Duncan氏新复极差法检验在0.05和0.01水平上差异显著。Note: Data in the table were shown as mean ± standard error. In the same column of data, different lowercase or capital letters indicated that there were significant differences at 0.05 and 0.01 levels, respectively.
    下载: 导出CSV

    表  2  紫外光照射时间对TraT2A抑菌活性的影响 (200.00 mg/mL)

    Table  2.   The influence of UV irradiation time on the inhibition activity of TraT2A (200.00 mg/mL)

    处理
    Treatment
    紫外光照射时间
    UV irradiation time/min
    抑制率
    Inhibition rate/%
    TraT2 A1059.68 ± 0.23 bB
    2045.70 ± 0.47 cC
    3029.57 ± 0.42 dD
    4020.61 ± 0.59 eE
    TraT2A 对照
    TraT2A control
    072.04 ± 0.21 aA
    CK0
    注:表中数据为平均值 ± 标准误差。同列数据后不同小写和大写字母分别表示经Duncan氏新复极差法检验在0.05和0.01水平上差异显著。Note: Data in the table were shown as mean ± standard error. In the same column of data, different lowercase or capital letters indicated that there were significant differences at 0.05 and 0.01 levels, respectively.
    下载: 导出CSV

    表  3  紫外保护剂对TraT2A抑菌活性的影响 (200.00 mg/mL)

    Table  3.   The influence of UV protective agents on the inhibition activity of TraT2A (200.00 mg/mL)

    处理
    Treatment
    紫外光照射时间
    UV irradiation time/min
    抑制率
    Inhibition rate/%
    TraT2A + 抗坏血酸
    TraT2A plus ascorbic acid
    30 54.41 ± 0.17 cC
    TraT2A + 腐殖酸
    TraT2A plus humic acid
    30 69.01 ± 0.67 bB
    TraT2 A 30 29.37 ± 0.41 dD
    TraT2 A 0 70.45 ± 0.57 aA
    抗坏血酸
    Ascorbic acid
    0 23.24 ± 0.48 eE
    腐殖酸
    Humic acid
    0 3.18 ± 0.43 fF
    CK 0
    注:表中数据为平均值 ± 标准误差。同列数据后不同小写和大写字母分别表示经Duncan氏新复极差法检验在0.05和0.01水平上差异显著。Note: Data in the table were shown as mean ± standard error. In the same column of data, different lowercase or capital letters indicated that there were significant differences at 0.05 and 0.01 levels, respectively.
    下载: 导出CSV

    表  4  辅助剂对TraT2A抑菌活性的影响 (200.00 mg/mL)

    Table  4.   The influence of adjuvants on the inhibition activity of TraT2A (200.00 mg/mL)

    处理
    Treatment
    浓度
    Concentration/(mg/mL)
    抑制率
    Inhibition rate/%
    辅助剂
    Adjuvant
    TraT2A + 辅助剂
    TraT2A + adjuvant
    与 TraT2A 对照相比抑菌作用提高百分点
    Increase percentage points of
    inhibition effect compared with TraT2A control
    IE-080.5015.56 ± 0.89 aA87.55 ± 0.53 aA18.36 aA
    0.2512.81 ± 0.61 bB81.46 ± 0.99 bB12.30 bB
    0.139.88 ± 0.36 cC76.74 ± 0.73 cC7.58 cC
    0.075.81 ± 0.82 dD74.44 ± 0.95 dD5.28 dD
    0.041.62 ± 0.94 eE70.24 ± 0.63 eE1.08 eE
    SP-4821 B0.1713.29 ± 0.60 aA88.61 ± 0.98 aA19.45 aA
    0.0910.96 ± 0.67 bB80.50 ± 0.61 bB11.34 bB
    0.059.28 ± 0.32 cC73.35 ± 0.44 cC4.19 cC
    0.036.77 ± 0.79 dD71.55 ± 0.51 cdCD2.39 cdCD
    0.023.41 ± 0.93 eE71.03 ± 0.46 deDE1.87 dD
    SP-4821 A0.1718.74 ± 0.18 aA95.13 ± 0.61 aA25.97 aA
    0.0914.84 ± 0.07 bB82.70 ± 0.69 bB13.54 bB
    0.0511.49 ± 0.89 cC75.10 ± 0.58 cC5.94 cC
    0.038.38 ± 1.01 dD73.33 ± 0.59 dD4.17 dD
    0.025.99 ± 0.99 eE70.59 ± 0.48 eE1.43 eE
    TraT2A 对照
    TraT2A control
    200.0069.16 ± 0.32 eE
    注:表中数据为平均值 ± 标准误差。同列数据后不同小写和大写字母分别表示经Duncan氏新复极差法检验在0.05和0.01水平上差异显著。
    Note: Data in the table were shown as mean ± standard error. In the same column of data, different lowercase or capital letters indicated that there were significant differences at 0.05 and 0.01 levels, respectively.
    下载: 导出CSV

    表  5  TraT2A与紫外保护剂、辅助剂混合液对百合叶斑病的田间防效

    Table  5.   The field control efficacy of TraT2A + UV protective agents + adjuvants mixture for lily leaf spot

    处理
    Treatment
    施药前病情
    指数
    Disease index
    before
    treatment
    第 1 次施药后 10 d
    Ten days after the
    first treatment
    第 2 次施药后 10 d
    Ten days after the
    second treatment
    第 3 次施药后 10 d
    Ten days after the
    third treatment
    病情
    指数
    Disease
    index
    防治
    效果
    Control
    efficacy/
    %
    病情
    指数
    Disease
    index
    防治
    效果
    Control
    efficacy/
    %
    病情
    指数
    Disease
    index
    防治
    效果
    Control
    efficacy/
    %
    200.00 mg/mL TraT2A + 5.00 mg/mL 腐殖酸 + 0.17 mg/mL SP-4821A
    200.00 mg/mLTraT2A + 5.00 mg/mL humic acid + 0.17 mg/mL SP-4821 A
    3.04 5.17 68.19 aA 5.81 75.41 aA 6.13 80.37 aA
    200.00 mg/mL TraT2 A 3.32 8.23 53.64 bB 10.04 61.08 bB 12.46 63.47 bB
    5.00 mg/mL腐殖酸
    5.00 mg/mL Humic acid
    3.09 15.33 7.22 dD 22.03 8.25 dD 28.97 8.75 dD
    0.17 mg/mL SP-4821 A 3.25 15.27 12.13 cC 21.76 13.84 cC 28.53 14.55 cC
    CK 3.14 16.79 24.40 32.26
    注:同列数据后不同小写和大写字母分别表示经Duncan氏新复极差法检验在0.05和0.01水平上差异显著。Note: In the same column of data, different lowercase or capital letters indicated that there were significant differences at 0.05 and 0.01 levels, respectively.
    下载: 导出CSV
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出版历程
  • 收稿日期:  2021-09-26
  • 录用日期:  2022-01-13
  • 网络出版日期:  2022-04-14
  • 刊出日期:  2022-08-03

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