• 百种中国杰出学术期刊
  • 中国精品科技期刊
  • 中国高校百佳科技期刊
  • 中国高校精品科技期刊
  • 中国国际影响力优秀学术期刊
  • 中国科技核心期刊

留言板

尊敬的读者、作者、审稿人, 关于本刊的投稿、审稿、编辑和出版的任何问题, 您可以本页添加留言。我们将尽快给您答复。谢谢您的支持!

姓名
邮箱
手机号码
标题
留言内容
验证码

虫螨腈及其代谢物溴代吡咯腈在芥菜上的残留行为与膳食风险评估

孙瑞卿 王霞 任鹏程 金静 李晋栋 乔雄梧 秦曙

孙瑞卿, 王霞, 任鹏程, 金静, 李晋栋, 乔雄梧, 秦曙. 虫螨腈及其代谢物溴代吡咯腈在芥菜上的残留行为与膳食风险评估[J]. 农药学学报, 2022, 24(3): 563-571. doi: 10.16801/j.issn.1008-7303.2022.0049
引用本文: 孙瑞卿, 王霞, 任鹏程, 金静, 李晋栋, 乔雄梧, 秦曙. 虫螨腈及其代谢物溴代吡咯腈在芥菜上的残留行为与膳食风险评估[J]. 农药学学报, 2022, 24(3): 563-571. doi: 10.16801/j.issn.1008-7303.2022.0049
SUN Ruiqing, WANG Xia, REN Pengcheng, JIN Jing, LI Jindong, QIAO Xiongwu, QIN Shu. Residue behavior and dietary risk assessment of chlorfenapyr and its metabolite tralopyril in mustard[J]. Chinese Journal of Pesticide Science, 2022, 24(3): 563-571. doi: 10.16801/j.issn.1008-7303.2022.0049
Citation: SUN Ruiqing, WANG Xia, REN Pengcheng, JIN Jing, LI Jindong, QIAO Xiongwu, QIN Shu. Residue behavior and dietary risk assessment of chlorfenapyr and its metabolite tralopyril in mustard[J]. Chinese Journal of Pesticide Science, 2022, 24(3): 563-571. doi: 10.16801/j.issn.1008-7303.2022.0049

虫螨腈及其代谢物溴代吡咯腈在芥菜上的残留行为与膳食风险评估

doi: 10.16801/j.issn.1008-7303.2022.0049
基金项目: 农业行业标准制定和修订项目.
详细信息
    作者简介:

    孙瑞卿,sunruiqing001@163.com

    通讯作者:

    秦曙,qinshu55@126.com

  • 中图分类号: TQ450.26;S482.3

Residue behavior and dietary risk assessment of chlorfenapyr and its metabolite tralopyril in mustard

Funds: Development and Revision of Agricultural Industry Standards.
  • 摘要: 为评价虫螨腈及其代谢物溴代吡咯腈在芥菜中的残留行为与膳食摄入风险,在山西、北京、吉林、河南、安徽、贵州6地进行了规范残留试验,建立了快速、简便的检测芥菜中虫螨腈残留的气相色谱-串联质谱(GC-MS/MS)方法及检测芥菜中溴代吡咯腈残留的超高效液相色谱-串联质谱(UPLC-MS/MS)方法,研究了虫螨腈及溴代吡咯腈在芥菜中的最终残留和消解动态,并就两种农药对中国不同人群的长期及短期膳食摄入风险进行了评估。结果表明:在0.01~30 mg/kg添加水平范围内,虫螨腈及溴代吡咯腈的平均回收率分别在89%~105%和97%~104%之间,相对标准偏差 (RSD) 分别在2%~4%和1%~3%之间;两种化合物在芥菜叶和根中的检测方法定量限 (LOQ) 均为0.01 mg/kg。虫螨腈在芥菜叶中的消解动态符合一级反应动力学方程,消解半衰期为4.2~5.9 d;溴代吡咯腈的消解动态因拟合曲线符合性太差无法进行一级反应动力学方程拟合。100 g/L虫螨腈悬浮剂以推荐最高剂量(有效成分105 g/hm2)施药2次、施药间隔期5 d、采收间隔期14 d,膳食消费量为97.5%位点处,芥菜叶中虫螨腈残留对3~5岁儿童和普通人群长期膳食摄入风险的贡献率分别为0.49%和2.47%,说明通过芥菜摄入虫螨腈及其代谢物残留对人体产生的长期膳食摄入风险较小;而短期膳食摄入风险评估结果表明,芥菜叶中虫螨腈对中国1~6岁儿童和普通人群均存在不可接受的短期膳食摄入风险,且对1~6岁儿童的风险远高于对普通人群。选择不同施药方式下的最终残留量数据进行评估,将可能导致风险评估结果产生较大差异,施药剂量或施药次数的增加会大幅提高农药残留的短期膳食摄入风险,建议可通过延长采收间隔期的方法降低农药的短期膳食摄入风险水平。
  • 图  1  虫螨腈母体在山西 (A) 和北京 (B) 两地芥菜叶中的消解曲线

    Figure  1.  Digestion curves of chlorfenapyr in mustard leaf in Shanxi (A) and Beijing (B)

    表  1  虫螨腈和溴代吡咯腈质谱参数

    Table  1.   MS parameters of chlorfenapyr and tralopyril

    化合物
    Compound
    保留时间
    Retention time/min
    定性离子对Qualitative ion, m/z定量离子对Quantitative ion, m/z碰撞能量
    Collision energy/eV
    锥孔电压
    Cone voltage/V
    虫螨腈 chlorfenapyr8.2246.9/227.0246.9/227.015
    136.9/102.015
    溴代吡咯腈 tralopyril3.0349.0/81.03330
    349.0/131.0349.0/131.03830
    下载: 导出CSV

    表  2  虫螨腈和溴代吡咯腈在芥菜中的添加回收率和相对标准偏差

    Table  2.   The recoveries and RSD of chlorfenapyr and tralopyril in mustard

    杀虫剂
    Insecticide
    基质
    Matrix
    标准曲线
    Standard curves
    添加水平
    Spiked level/(mg/kg)
    平均回收率
    Average recovery/%
    相对标准偏差
    RSD/%
    虫螨腈 chlorfenapyr 芥菜根 Mustard root y = 538617x − 4344.5 R2 = 0.9985 0.01 93 4
    0.1 102 2
    1 105 4
    芥菜叶 Mustard leaf y = 808495x − 567.18 R2 = 0.9998 0.01 98 3
    0.1 100 3
    30 89 4
    溴代吡咯腈 tralopyril 芥菜根 Mustard root y = 416666x + 2985.6 R2 = 0.9975 0.01 97 2
    0.05 102 1
    0.1 97 1
    芥菜叶 Mustard leaf y = 397554x + 3742.5R2 = 0.9949 0.01 104 3
    0.1 103 2
    0.3 97 2
    下载: 导出CSV

    表  3  芥菜中虫螨腈、溴代吡咯腈及虫螨腈总量(评估定义)的最终残留

    Table  3.   Final residues of chlorfenapyr, tralopyril and total chlorfenapyr (evaluation definition) in mustard

    基质
    Matrix
    施药次数
    Times of application
    施药剂量
    Dosage, a.i./(g/hm2)
    采收间隔期
    Interval to harvest/d
    残留量
    Residue/(mg/kg)
    虫螨腈
    chlorfenapyr
    溴代吡咯腈
    tralopyril
    虫螨腈总量 (风险评估定义)Total chlorfenapyr
    (definition for risk assessment)
    芥菜根
    Mustard root
    210500.032~0.17<0.0100.032~0.17
    100.026~0.21<0.0100.026~0.21
    140.015~0.22<0.0100.015~0.22
    210.019~0.18<0.0100.019~0.18
    2157.500.045~0.48<0.0100.045~0.48
    100.042~0.74<0.0100.042~0.74
    140.039~0.36<0.0100.039~0.36
    210.031~0.45<0.0100.031~0.45
    310500.036~0.42<0.0100.036~0.42
    100.014~0.20<0.0100.014~0.20
    140.034~0.28<0.0100.034~0.28
    210.030~0.23<0.0100.030~0.23
    3157.500.048~0.86<0.0100.048~0.86
    100.034~0.67<0.0100.034~0.67
    140.064~0.65<0.0100.064~0.65
    210.033~0.58<0.0100.033~0.58
    芥菜叶
    Mustard leaf
    210501.79~7.74<0.010~0.152.14~8.50
    100.46~4.17<0.010~0.170.46~5.63
    140.25~6.80<0.010~0.120.25~7.97
    210.20~4.59<0.010~0.0660.20~5.25
    2157.502.03~14.060.020~0.172.23~15.03
    100.23~10.50<0.010~0.260.23~13.06
    140.24~8.40<0.010~0.210.24~10.52
    210.14~2.04<0.010~0.170.14~3.25
    310501.15~12.060.035~0.131.66~12.99
    100.36~8.51<0.010~0.220.36~10.35
    140.20~8.72<0.010~0.190.20~10.58
    210.11~1.27<0.010~0.0860.11~1.94
    3157.502.94~29.56<0.010~0.162.94~29.95
    100.70~17.47<0.010~0.380.70~21.07
    140.31~22.59<0.010~0.340.31~25.98
    210.080~2.71<0.010~0.220.080~4.87
    下载: 导出CSV

    表  4  虫螨腈母体在芥菜叶中的消解参数

    Table  4.   Dissipation parameters for chlorfenapyr in the mustard leaf

    农药
    Pesticide
    试验地点
    Location
    消解方程
    Digestion equation
    相关系数
    r
    半衰期
    T1/2/d
    k/(d−1)
    虫螨腈
    chlorfenapyr
    山西 Shanxict = 5.316 8e−0.118t0.94455.90.118
    北京 Beijingct = 2.706 5e−0.163t0.96304.20.163
    下载: 导出CSV

    表  5  芥菜叶中虫螨腈残留的长期膳食摄入风险评估结果[19]

    Table  5.   The results of long-term dietary intake risk assessment of chlorfenapyr in mustard leaf[19]

    消费群体
    Consumer group
    膳食 50% 位点
    50% food comsumption
    膳食 95% 位点
    95% food comsumption
    膳食 97.5% 位点
    97.5% food comsumption
    膳食消费量
    Dietary consumption/(g/d)
    RQc% 膳食消费量
    Dietary consumption/(g/d)
    RQc% 膳食消费量
    Dietary consumption/(g/d)
    RQc%
    一般人群
    General population
    1.8939 0.12 6.25 0.40 7.6923 0.49
    3~5 岁儿童
    3-5 years children
    3.3109 0.64 10.5556 2.03 12.8 2.47
    注:膳食消费量/体重的计算单位:g/kg bw。 Note: Unit of measure for dietary consumption/ body weight: g/ kg bw.
    下载: 导出CSV

    表  6  虫螨腈的短期膳食摄入风险评估结果

    Table  6.   The results of short-term dietary intake risk assessment of chlorfenapyr

    情景
    Scenario
    施药次数Times of
    application
    施药剂量Dosage/
    (g/hm2)
    采收间隔期Interval to
    harvest/d
    芥菜叶中残留量 (总量计)
    Residue of mustard leaf (total)/(mg/kg)
    STMR/
    (mg/kg)
    HR/
    (mg/kg)
    %ARfD
    1~6 岁儿童Aged1-6
    children
    普通人群General
    population
    1 2 105 14 0.25,0.29,0.36,0.47,0.48,0.70,0.75,0.78,0.96,1.07,1.21,1.41,1.54,1.54,1.64,5.23,5.54,7.97 1.02 7.97 1299 521
    2 2 157.5 14 0.24,0.28,0.55,0.97,0.99,1.03,1.10,1.31,1.47,1.91,2.54,2.79,2.80,3.65,4.06,8.25,8.90,10.52 1.69 10.52 1715 688
    3 3 105 14 0.20,0.22,0.26,059,0.62,0.72,0.74,0.78,1.03,1.08,1.47,1.79,2.69,3.04,3.08,9.16,9.34,10.58 1.06
    10.58 1725 692
    4 2 105 21 0.20,0.22,0.22,0.33,0.35,0.41,0.44,0.54,0.56,0.64,0.66,0.73,0.75,0.84,1.06,3.77,3.81,5.25 0.60 5.25 856 343
    下载: 导出CSV
  • [1] 李哲, 徐冬梅, 张兰, 等. QuEChERS-超高效液相色谱法测定水样中吡虫啉和虫螨腈[J]. 农药, 2019, 58(4): 291-292,299.

    LI Z, XU D M, ZHANG L, et al. Determination of imidacloprid and chlorfenapyr in water by QuEChERS and UPLC[J]. Agrochemicals, 2019, 58(4): 291-292,299.
    [2] 伍灿. 虫螨腈环境安全性评价[D]. 长沙: 湖南农业大学, 2016: 2-3.

    WU C. Environmental safety evaluation for chlorfenapyr[D]. Changsha: Hunan Agricultural University, 2016: 2-3.
    [3] 中国农业信息网[DB/OL]. [2020-12-27]. http://www.chinapesticide.org.cn/.

    China pesticide information network [DB/OL]. [2020-12-27]. http://www.chinapesticide.org.cn/.
    [4] FAO, WHO. Pesticide residues in food 2018-Report 2018-Joint FAO/WHO Meeting on Pesticide Residues[M]. Rome: FAO and WHO, 2019: 39-55.
    [5] 李晓贝, 赵晓燕, 李健英, 等. 阿维菌素 • 虫螨腈在青菜上的残留特性及其膳食摄入风险评估[J]. 食品安全质量检测学报, 2020, 11(13): 4185-4190.

    LI X B, ZHAO X Y, LI J Y, et al. Residue behavior and dietary intake risk assessment of abamectin and chlorfenapyr in pakchoi(Brassica chinensis L.)[J]. J Food Saf Qual, 2020, 11(13): 4185-4190.
    [6] 冯义志, 梁林, 张爱娟, 等. 虫螨腈在姜中的残留量及消解动态研究[J]. 农药科学与管理, 2020, 41(2): 21-24. doi: 10.3969/j.issn.1002-5480.2020.02.006

    FENG Y Z, LIANG L, ZHANG A J, et al. Residue and decline study of chlorfenapyr in ginger[J]. Pesticide Science and Administration, 2020, 41(2): 21-24. doi: 10.3969/j.issn.1002-5480.2020.02.006
    [7] 王兵, 李斌, 张媛媛, 等. QuEChERS-高效液相色谱法测定绿茶中虫螨腈残留[J]. 中国食品学报, 2017, 17(1): 240-245.

    WANG B, LI B, ZHANG Y Y, et al. Determination of chlorfenapyr in green tea by QuEChERS-high-performance liquid chromatography[J]. Journal of Chinese Institute of Food Science and Technology, 2017, 17(1): 240-245.
    [8] 伍灿, 刘一平, 熊兴明, 等. 虫螨腈在大白菜和土壤中的残留降解研究[J]. 湖南农业科学, 2016(4): 72-75.

    WU C, LIU Y P, XIONG X M, et al. Study on residues and dissipation of chlorfenapyr in Chinese cabbage and soil[J]. Hunan Agricultural Sciences, 2016(4): 72-75.
    [9] 吴迪, 罗雪婷, 潘洪吉, 等. 气相色谱法测定黄瓜、苹果中的虫螨腈残留量[J]. 食品科学, 2010, 31(10): 268-271.

    WU D, LUO X T, PAN H J, et al. GC determination of chlorfenapyr residue in cucumbers and apples[J]. Food Sci, 2010, 31(10): 268-271.
    [10] 袁一宸. 虫螨腈在猕猴桃上的残留风险评估[D]. 合肥: 安徽农业大学, 2019.

    YUAN Y C. Residue risk assessment of chlorfenapyr on Actinidia chinensis[D]. Hefei: Anhui Agricultural University, 2019.
    [11] 孙丰收, 罗媛媛, 张文文, 等. 甘蓝中虫螨腈及其代谢物的残留检测[J]. 农药科学与管理, 2020, 41(7): 37-41.

    SUN F S, LUO Y Y, ZHANG W W, et al. Determination of residues of tetranylonitrile and its metabolites in cabbage[J]. Pestic Sci Adm, 2020, 41(7): 37-41.
    [12] 冯义志, 李瑞娟, 王晓玉, 等. 田间条件下虫螨腈在菜苔中的残留消解及膳食风险评估[J]. 农药, 2020, 59(9): 665-669.

    FENG Y Z, LI R J, WANG X Y, et al. Residue dissipation and dietary risk assessment of chlorfenapyr in flowering Chinese cabbage under field conditions[J]. Agrochemicals, 2020, 59(9): 665-669.
    [13] 农作物中农药残留试验准则: NY/T 788—2018[S]. 北京: 中国农业出版社, 2018.

    Guideline for the testing of pesticide residues in crops: NY/T 788—2018[S]. Beijing: China Agriculture Press, 2018.
    [14] 中国农业科学院农产品质量标准与检测技术研究所. 农产品质量安全风险评估: 原理、方法和应用[M]. 北京: 中国标准出版社, 2007.

    Institute of Quality Standards and Testing Technology for Agroproducts, Chinese Academy of Agricultural Sciences. Risk assessment for quality and safety of agro-foods: principles, methodologies and applications[M]. Beijing: Standards Press of China, 2007.
    [15] Food and Agriculture Organization of the United Nations (FAO). Report of the joint meeting of the FAO panel of experts on pesticide residues in food and the environment and the WHO core assessment group on pesticide residue [DB/OL]. [2019-12-20]. https://www.who.int/foodsafety/areas_work/chemical-risks/gems-food/en/.
    [16] 食品安全国家标准 食品中农药最大残留限量: GB 2763—2021[S]. 北京: 中国标准出版社, 2021.

    National food safety stanard, maximum residue limits for pesticides in food: GB 2763—2019[S]. Beijing: Standards Press of China, 2019.
    [17] 常继乐, 王宇. 2010-2013 年中国居民营养与健康状况监测综合报告[M]. 北京: 北京大学医学出版社, 2016: 61.

    CHANG J L, WANG Y. Comprehensive report of nutrition and health status for China residents of 2010-2013[M]. Beijing: Peking University Medical Press, 2016: 61.
    [18] Food and Agriculture Organization of the United Nations (FAO). Report of the joint meeting of the FAO panel of experts on pesticide residues in food and the environment and the WHO core assessment group on pesticide residue[DB/OL]. [2021-9-30]. https://www.who.int/foodsafety/areas_work/chemical-risks/gems-food/en/
    [19] Food and Agriculture Organization of the United Nations. AGP-D list of pesticide evaluated by JMPR and JMPS-D[DB/OL][2019-10-13]. http://www.fao.org/agriculture/crops/core-themes/theme/pests/lpe/en/.
    [20] 孙瑞卿, 乔雄梧, 秦曙. 中国食品中农药MRL标准对短期膳食暴露评估需求的研究[J]. 农药学学报, 2015, 17(5): 544-554. doi: 10.3969/j.issn.1008-7303.2015.05.07

    SUN R Q, QIAO X W, QIN S. Study on acute dietary exposure assessment needs for Chinese pesticide MRLs in food[J]. Chin J Pestic Sci, 2015, 17(5): 544-554. doi: 10.3969/j.issn.1008-7303.2015.05.07
    [21] 乔雄梧. 食品中农药残留膳食暴露评估若干问题探讨[J]. 农药学学报, 2020, 22(5): 727-733.

    QIAO X W. Some considerations on dietary exposure assessment of pesticide residues in food[J]. Chin J Pestic Sci, 2020, 22(5): 727-733.
  • 加载中
图(1) / 表(6)
计量
  • 文章访问数:  101
  • HTML全文浏览量:  21
  • PDF下载量:  32
  • 被引次数: 0
出版历程
  • 收稿日期:  2021-11-19
  • 录用日期:  2022-04-11
  • 网络出版日期:  2022-05-26
  • 刊出日期:  2022-06-10

目录

    /

    返回文章
    返回