根结线虫趋化性研究进展

王帅; 魏钰洋; 张羲; 谢佳; 胡展; 孙然锋

doi:10.16801/j.issn.1008-7303.2022.0090

根结线虫趋化性研究进展

doi: 10.16801/j.issn.1008-7303.2022.0090

王帅^{1, 2,},
魏钰洋^{1, 2},
张羲^{1, 2},
谢佳^{1, 2},
胡展^{1, 2},
孙然锋^{1, 2, ,}

1.
海南大学热带农林生物灾害绿色防控教育部重点实验室，海口 570228
2.
海南大学植物保护学院，海口 570228

基金项目: 国家自然科学基金 (22067004)；海南省自然科学基金资助 (321CXTD436, 321QN177, 321QN178)；海南省烟草公司科技计划(2018003).

详细信息

作者简介:
王帅，996018@hainanu.edu.cn

通讯作者:
孙然锋，srf18@hainanu.edu.cn

中图分类号: S482.51；TQ459
计量
- 文章访问数: 92
- HTML全文浏览量: 30
- PDF下载量: 41
- 被引次数: 0
出版历程
- 收稿日期: 2022-07-20
- 录用日期: 2022-08-23
- 网络出版日期: 2022-09-16
- 刊出日期: 2022-10-10

Advances in studies on the chemotaxis of root-knot nematodes

WANG Shuai^{1, 2
,},
WEI Yuyang^{1, 2},
ZHANG Xi^{1, 2},
XIE Jia^{1, 2},
HU Zhan^{1, 2},
SUN Ranfeng^{1, 2
, ,}

1.
Key Laboratory of Green Prevention and Control of Tropical Agriculture and Forestry Biological Disasters Ministry of Education, Hainan University, Haikou 570228, China
2.
College of Plant Protection, Hainan University, Haikou 570228, China

Funds: National Natural Science Foundation of China (22067004); Hainan Provincial Natural Science Foundation of China(321CXTD436, 321QN177, 321QN178); Annual Science and Technology Project of Hainan Provincial Tobacco Company of CNTC (2018003).

摘要

摘要: 植物寄主根部及其根际微生物等释放的化学信号物质在根结线虫寻找寄主、配偶及逃避危险等行为中起着重要的作用。目前，明确根结线虫预侵染阶段的化学信号物质以及相关的分子靶标，以期开发得到植物源和微生物源引诱剂及驱避剂，是国际线虫学研究领域的前沿和热点。本文重点综述了根结线虫的趋化性及化学信号物质，概括了线虫诱饵效应与诱捕现象的研究进展，概述了平面琼脂及其改进模型、Pluronic凝胶模型以及沙土/土壤模型在根结线虫趋化性测试中的应用，讨论了根结线虫趋化性研究的意义和难度，并对未来根结线虫趋化性研究的主要方向及前景进行了总结和展望。
- 根结线虫 /
- 趋化性 /
- 化学信号物质 /
- 诱捕现象 /
- 趋化性模型 /
- 引诱剂 /
- 驱避剂
Abstract: Allelochemical signaling substances released by host roots and rhizosphere microbes play an important role in the host-seeking, mate-searching, and danger escape of root-knot nematodes (RKNs). At present, in order to develop chemoattractants and repellents of plant and microbial sources, it is an international hotspot and frontier for nematologists to identify semiochemicals and related molecular targets in the pre-infection stage of RKNs. This review focused on the chemotaxis and allelochemicals of RKNs, including attractive and repellent compounds, and summarized the progress on Trojan horse mechanism and trapping formation of RKNs. Furthermore, the application of the chemotactic models, including the plate agar and its modified model, Pluronic gel model, and the sand/soil model, on the nematodes chemotaxis test were summarized. Finally, the significance and challenge of the research on RKNs chemotaxis were discussed, and research directions on RKNs chemotaxis in the future were prospected.
- root-knot nematode /
- chemotaxis /
- semiochemicals /
- nematode-trapping formation /
- chemotactic model /
- chemoattractants /
- repellents

HTML全文

图 1 测试线虫趋化性的两种琼脂模型

a：培养皿做成的琼脂模型^[23]；b：含微孔装置的琼脂模型^[25]。

Figure 1. Two kind of agar models for testing nematode chemotaxis

a: Agar model made by petri dish^[23]; b: Agar model with microchannel device^[25].

下载: 全尺寸图片幻灯片

图 2 测试线虫趋化性的普朗尼克凝胶模型

a：单选择的普朗尼克凝胶模型^[18]；b：具有双选择的普朗尼克凝胶模型^[15]。

Figure 2. Pluronic F127 gel models for testing nematode chemotaxis

a: Pluronic F127 gel models with single-choice^[18]; b: Pluronic F127 gel models with dual-choice^[15].

下载: 全尺寸图片幻灯片

图 3 测试线虫趋化性的沙土/土壤模型

a：育苗盆与管制做的沙土/土壤模型^[22]；b：培养皿和试管制做的沙土/土壤模型^[29]。

Figure 3. Sandy/soil models for testing nematode chemotaxis

a: Sandy/soil models made by pots and tubes^[22]; b: Sandy/soil models made by petri dishes and tubes^[29].

下载: 全尺寸图片幻灯片

表 1 对根结线虫具有引诱作用的植物源化合物

Table 1. Plant-derived compounds with attractant properties toward root-knot nematodes

化合物 Compound	来源植物 Plant source	模型种类 (活性浓度)，用量 Model type (active concentration), dosage	报道时间/ 线虫种类 Published time/ Nematode species	化学结构 Chemical structure
α-蒎烯 α-Pinene	辣椒 Capsicum annum	沙土/土壤 Sand/soil model (20~80 ng/μL)，2 mL^[12]	2017/ M. incognita
柠檬烯 Limonene	辣椒 C. annum	沙土/土壤 Sand/soil model (20~80 ng/μL)，2 mL^[12]	2017/ M. incognita
2-甲氧基-3-(1-甲基丙基)-吡嗪 2-Methoxy-3-(1-methylpropyl)-pyrazine	辣椒 C. annum；番茄 Solanum lycopersicum；菠菜 Spinacea oleracea	沙土/土壤 Sand/soil model (>80 ng/μL)，2 mL^[12]；沙土/土壤 Sand/soil model (>160 ng/μL)，50 μL^[13]	2017, 2018/ M. incognita
水杨酸甲酯 Methyl salicylate	辣椒 C. annum；番茄 S. lycopersicum	沙土/土壤 Sand/soil model (20~80 ng/μL)，2 mL^[12]；沙土/土壤 Sand/soil model (40~160 ng/μL)，50 μL^[13]；普朗尼克凝胶 Pluronic gel (50~200 μg/mL)，1 mL^[15]	2017, 2018, 2020/ M. incognita
十三烷 Tridecane	辣椒 C. annum；番茄 S. lycopersicum；菠菜 S. oleracea	沙土/土壤 Sand/soil model (40~80 ng/μL)，2 mL^[12]；沙土/土壤 Sand/soil model (80~160 ng/μL)，50 μL^[13]； 1% 琼脂 1% agar (10⁻³~10² mg/mL)，10 μL^[17]	2017, 2018, 2021/ M. incognita
2-异丙基-3-甲氧基吡嗪 2-Isopropyl-3 methoxy-pyrazine	菠菜 S. oleracea	沙土/土壤 Sand/soil model (40~160 ng/μL)，50 μL^[13]	2018/ M. incognita
玉米素 Zeatin (cytokinin)	番茄 S. lycopersicum	沙土/土壤 Sand/soil model (16~4000 μg/g ) ^[14]	2018/ M. incognita
木樨草素 Luteolin	番茄 S. lycopersicum	沙土/土壤 Sand/soil model (16 μg/g) ^[14]	2018/ M. incognita
槲皮素 Quercetin	番茄 S. lycopersicum	沙土/土壤 Sand/soil model (16~249.2 μg/g) ^[14]	2018/ M. incognita
番茄碱 Tomatine	番茄 S. lycopersicum	沙土/土壤 Sand/soil model (16~62.4 μg/g) ^[14]	2018/ M. incognita
茄碱 Solasodine	番茄 S. lycopersicum	沙土/土壤 Sand/soil model (16 μg/g沙土) ^[14]	2018/ M. incognita
L-抗坏血酸-2,6-二棕榈酸酯 L-Ascorbyl 2,6-dipalmitate	番茄 S. lycopersicum	普朗尼克凝胶 Pluronic gel (50~200 μg/mL)，1 mL^[15]	2020/ M. incognita
邻苯二甲酸二丁酯 Butyl phthalate	番茄 S. lycopersicum	普朗尼克凝胶 Pluronic gel (50~200 μg/mL)，1 mL^[15]	2020/ M. incognita
棕榈酸 Palmitic acid	番茄 S. lycopersicum	普朗尼克凝胶 Pluronic gel (50~200 μg/mL)，1 mL^[15]	2020/ M. incognita
壬二酸 Azelaic acid	番茄 S.lycopersicum	普朗尼克凝胶 Pluronic gel (50~200 μg/mL)，1 mL^[15]	2020/ M. incognita
癸二酸 Sebacic acid	番茄 S. lycopersicum	普朗尼克凝胶 Pluronic gel (50~200 μg/mL)，1 mL^[15]	2020/ M. incognita
对羟基苯甲酸 4-Hydroxybenzoic acid	番茄 S. lycopersicum	普朗尼克凝胶 Pluronic gel (50~100 μg/mL)，1 mL^[15]	2020/ M. incognita
肉桂酸 Cinnamic acid	番茄 S. lycopersicum	普朗尼克凝胶 Pluronic gel (50~200 μg/mL)，1 mL^[15]	2020/ M. incognita
阿魏酸 Ferulic acid	番茄 S. lycopersicum	普朗尼克凝胶 Pluronic gel (50~200 μg/mL)，1 mL^[15]	2020/ M. incognita
β-蒎烯 β-Pinene	番茄 S. lycopersicum	沙土/土壤 Sand/soil model (55, 110 ng/μL)，50 μL^[16]	2020/ M. javanica
(+)-(2)-蒈烯 (+)-(2)-Carene	番茄 S. lycopersicum	沙土/土壤 Sand/soil model (682 ng/μL)，50 μL^[16]	2020/ M. javanica
α-水芹烯 α-Phellandrene	番茄 S. lycopersicum	沙土/土壤 Sand/soil model (110~220 ng/μL)，50 μL^[16]	2020/ M. javanica
β-水芹烯 β-Phellandrene	番茄 S. lycopersicum	沙土/土壤 Sand/soil model (>220 ng/μL)，50 μL^[16]	2020/ M. javanica
α-L-半乳糖-1,3-L-鼠李糖 α-L-Galactosyl-1,3-L-rhamnose	亚麻 Linum usitatissimum	普朗尼克凝胶 Pluronic gel (>150 mmol/L)，1 μL^[5]	2021/ M. incognita
1-二十二碳烯 1-Docosene	小白菜 Brassica chinensis；番茄 S. lycopersicum；萝卜等 Raphanus sativus; et al	1% 琼脂 1% agar (10⁻³ mg/mL)，10 μL^[17]	2021/ M. incognita
1-十八烯 1-Octadecene	小白菜 B. chinensis；番茄 S. lycopersicum；萝卜等 R. sativus; et al	1% 琼脂 1% agar (10⁻⁶~10² mg/mL)，10 μL^[17]	2021/ M. incognita
2-己基-1-癸醇 2-Hexyl-1-decanol	小白菜 B. chinensis；番茄 S. lycopersicum；黄瓜 Cucumis sativus	1% 琼脂 1% agar (10⁻⁶~10² mg/mL)，10 μL^[17]	2021/ M. incognita
苹果酸 Malic acid	小白菜 B. chinensis；番茄 S. lycopersicum；小米椒等 Capsicum frutescens; et al	1% 琼脂 1% agar (10⁻²~10² mg/mL)，10 μL^[17]	2021/ M. incognita
草酸 Oxalic acid	小米椒 C. frutescens；萝卜等 R. sativus; et al	1% 琼脂 1% agar (10⁻²~10⁻¹ mg/mL)，10 μL^[17]	2021/ M. incognita
乳酸 Lactic acid	小白菜 B. chinensis；番茄 S. lycopersicum；萝卜等 R. sativus; et al	1% 琼脂 1% agar (10⁻²~10² mg/mL)，10 μL^[17]	2021/ M. incognita

下载: 导出CSV

表 2 对根结线虫具有引诱作用的微生物源化合物

Table 2. Microbial compounds with attractant properties toward root-knot nematodes

化合物 Compound	来源微生物 Microbial source	模型种类 (活性浓度)，用量 Model type (active concentration), dosage	报道时间/线虫种类 Published time/Nematode species	化学结构 Chemical structure
二苯并呋喃 Dibenzofuran	链霉菌 Streptomyces plicatus	沙土/土壤 Sand/soil model (10~30 μg/g) ^[22]	2019/ M. incognita
乙基苯 Ethylbenzene	独岛枝芽胞杆菌 Virgibacillus dokdonensis	2% 琼脂 2% agar (1~10 mg/mL)，30 μL^[23]	2020/ M. incognita
二甲基二硫醚 Dimethyl disulfide	独岛枝芽胞杆菌 V. dokdonensis	2% 琼脂 2% agar (1~10 mg/mL)，30 μL^[23]	2020/ M. incognita
乙醛 Acetaldehyde	独岛枝芽胞杆菌 V. dokdonensis	2% 琼脂 2% agar (1~10 mg/mL)，30 μL^[23]	2020/ M. incognita
糠醛丙酮 Furfural acetone	多粘类芽孢杆菌 Paenibacillus polymyxa	普朗尼克凝胶 Pluronic gel (20~60 mg/mL)，30 μL^[24]	2021/ M. incognita

下载: 导出CSV

表 3 对根结线虫具有引诱作用的合成类化学物

Table 3. Synthetic chemicals with attractant properties toward root-knot nematodes

化合物 Compound	模型种类 (活性浓度)，用量 Model type (active concentration), dosage	报道时间/线虫种类 Published time/ Nematode specie	化学结构 Chemical structure
1,3-二氨基丙烷 1,3-Diaminopropane	普朗尼克凝胶 Pluronic gel (100 mmol/L)，1 μL^[28]	2020/M. incognita
腐胺 Putrescine	普朗尼克凝胶 Pluronic gel (100 mmol/L)，1 μL^[28]	2020/M. incognita
尸胺 Cadaverine	普朗尼克凝胶 Pluronic gel (100 mmol/L)，1 μL^[28]	2020/M. incognita
2-甲氧基肉桂醛 2-Methoxycinnamaldehyde	沙土/土壤 Sand/soil model (10 mg/mL)，10 μL^[29]	2021/M. incognita, M. javanica, M. marylandi, M. hapla
2-羟基苯甲酸 2-Hydroxybenzoic acid (salicylic acid)	沙土/土壤 Sand/soil model (10 mg/mL)，10 μL^[29]	2021/M. incognita, M. javanica, M. marylandi
2-羟基-3-甲氧基苯甲醛 2-Hydroxy-3-methoxybenzaldehyde	沙土/土壤 Sand/soil model (10 mg/mL)，10 μL^[29]	2021/M. marylandi, M. hapla
3-甲氧基苯甲酸 3-Methoxybenzoic acid	沙土/土壤 Sand/soil model (10 mg/mL)，10 μL^[29]	2021/M. incognita, M. javanica, M. marylandi, M. hapla
4-甲氧基苯甲酸 4-Methoxybenzoic acid	沙土/土壤 Sand/soil model (10 mg/mL)，10 μL^[29]	2021/M. incognita, M. javanica, M. marylandi, M. hapla
反式肉桂酸 Trans-cinnamic acid	沙土/土壤 Sand/soil model (10 mg/mL)，10 μL^[29]	2021/M. incognita, M. javanica

下载: 导出CSV

表 4 对根结线虫具有驱避作用的植物源化合物

Table 4. Plant-derived compounds with repellent properties toward root-knot nematodes

化合物 Compound	来源植物 Plant source	模型种类 (活性浓度)，用量 Model type (active concentration), dosage	报道时间/线虫种类 Published time/ Nematode species	化学结构 Chemical structure
月桂酸 Lauric acid	冠雏菊 Chrysanthemum coronarium	2% 琼脂 2% agar (4 mmol/L)，10 μL^[33]	2014/ M. incognita
百里香酚 Thymol	辣椒 C. annum	沙土/土壤 Sand/soil model (40~80 ng/μL)，2 mL^[12]	2017/ M. incognita
棕榈酸 Palmitic acid	蓖麻 Ricinus communis	2% 琼脂 2% agar (0.5~4.0 mmol/L)，50 μL^[34]	2018/ M. incognita
亚油酸 Linoleic acid	蓖麻 R. communis	2% 琼脂 2% agar (0.5~4.0 mmol/L)，50 μL^[34]	2018/ M. incognita
棕榈酸甲酯 Methyl palmitate	木薯 Manihot esculenta	1% 琼脂 1% agar (1~16 mmol/kg)，20 μL^[35]	2020/ M. incognita
硬脂酸甲酯 Methyl stearate	木薯 M. esculenta	1% 琼脂 1% agar (1~16 mmol/kg)，20 μL^[35]	2020/ M. incognita
二氢辣椒碱 Dihydrocapsaicin	番茄 S. lycopersicum	普朗尼克凝胶 Pluronic gel (100~200 μg/mL)，1 mL^[15]	2020/ M. incognita
澳洲茄胺 Solasodine	番茄 S. lycopersicum	普朗尼克凝胶 Pluronic gel (100~200 μg/mL)，1 mL^[15]	2020/ M. incognita
1-二十二碳烯 1-Docosene	小白菜 B. chinensis；番茄 S. lycopersicum；萝卜等 R. sativus; et al	1% 琼脂 1% agar (10⁻²~10² mg/mL)，10 μL^[17]	2021/ M. incognita

下载: 导出CSV

表 5 对根结线虫具有驱避作用的微生物源化合物

Table 5. Microbial compounds with repellent properties toward root-knot nematodes

化合物 Compound	来源微生物 Microbial source	模型种类 (活性浓度)，用量 Model type (active concentration), dosage	报道时间/线虫种类 Published time/ Nematode species	化学结构 Chemical structure
二甲基二硫醚 Dimethyl-disulfide	恶臭假单胞菌 Pseudomonas putida	2% 琼脂 2% agar (1~10000 mg/L) ^[37]	2018/ M. incognita
1-十一烯 1-Undecene	恶臭假单胞菌 P. putida	2% 琼脂 2% agar (1~10000 mg/L) ^[37]	2018/ M. incognita
2-壬酮 2-Nonanone	恶臭假单胞菌 P. putida	2% 琼脂 2% agar (1~10000 mg/L) ^[37]	2018/ M. incognita
2-辛酮 2-Octanone	恶臭假单胞菌 P. putida	2% 琼脂 2% agar (1~10000 mg/L) ^[37]	2018/ M. incognita
(Z)-己烯-1-乙酸醇 (Z)-Hexen-1-ol acetate	恶臭假单胞菌 P. putida	2% 琼脂 2% agar (1~10000 mg/L) ^[37]	2018/ M. incognita
2-十一酮 2-Undecanone	恶臭假单胞菌 P. putida	2% 琼脂 2% agar (1~10000 mg/L) ^[37]	2018/ M. incognita
1-(乙烯氧基)-十八烷 1-(Ethenyloxy)-octadecane	恶臭假单胞菌 P. putida	2% 琼脂 2% agar (1~10000 mg/L) ^[37]	2018/ M. incognita
苯并噻唑 Benzothiazole	链霉菌 S. plicatus	沙土/土壤 Sand/soil model (10~30 μg/g) ^[22]	2019/ M. incognita
2-丁酮 2-Butanone	独岛枝芽胞杆菌 V. dokdonensis	2% 琼脂 2% agar (1~10 mg/mL)，30 μL^[23]	2020/ M. incognita

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