Abstract: In this study, Trichoderma asperellum TCS007, isolated from marine habitat, was evaluated for its ability to promote growth and induce stress tolerance in plants. Experiments were conducted to test the effects of TCS007 on seed germination, seedling and root growth, and the content of chlorophyll, soluble sugar and protein in cucumber and rice. In addition, the key physiological indicators of TCS007 treated and untreated cucumber and rice plants were measured under low temperature and high salt stresses. The results showed that 1 × 10⁶ CFU/mL spore suspension of TCS007 significantly promoted cucumber seed germination. Plant fresh weight, leaf area, root fresh weight, and root activity of treated cucumber seedlings increased by 13.53%, 17.97%, 66.67%, and 27.30%, respectively. The contents of total chlorophyll, soluble sugar, and soluble protein increased by 22.75%, 18.24%, and 8.60%, respectively. The activities of peroxidase (POD) and superoxide dismutase (SOD) increased and malondialdehyde (MDA) and the relative conductivity (REC) content decreased in cucumber leaves treated with 1 × 10⁶ CFU/mL spore suspension of TCS007 at 5 ℃, 10 ℃, and 15 ℃. Under 0.1 mol/L NaCl (high salt) stress, TCS007 significantly alleviated the salt damage to rice seedlings. The alleviating effect increased as the concentration of TCS007 spore suspension increased. The activities of POD, SOD, and catalase (CAT) were significantly increased, and REC was decreased in rice seedlings treated with 5% 1 × 10⁶ CFU/mL TCS007 spore suspension. Overall, the study suggests T. asperellum TCS007 has positive effects on plant growth and stress tolerances and provides a theoretical basis for its development as a biopesticide.