直接竞争酶联免疫吸附分析法测定桃中氰戊菊酯的残留量

Determination of fenvalerate residue in peach by direct competitive enzyme-linked immunosorbent assay

摘要: 采用包被抗体、酶标半抗原直接竞争酶联免疫吸附分析法（ELISA）测定了氰戊菊酯在桃中的残留量。结果表明：在氰戊菊酯多克隆抗体包被浓度为4.0 mg/L、辣根过氧化物酶（HRP）标记氰戊菊酯半抗原稀释1.0×105倍条件下，ELISA法检测氰戊菊酯的线性浓度范围为0.01~10 mg/L，氰戊菊酯对抗体-酶标半抗原反应的抑制中浓度（IC50）为193 μg/L，相对标准偏差（RSD，n=5）为4.5%，IC20为13.5 μg/L。在2、0.2和0.05 mg/kg添加水平下，ELISA法测定桃中氰戊菊酯的回收率分别为81%~89%、85%~98%和85%~106%，RSD（n=5）分别为5.1%、5.6%和7.7%。ELISA法对桃中氰戊菊酯的最低检出浓度为0.014 mg/kg。

Abstract: The method of antibody immobilized direct competitive enzyme-linked immunosorbent assay (ELISA) was employed to determine fenvalerate residue in peach. The result showed that the linear concentration of ELISA ranged from 0.01 to 10 mg/L, the half maximal inhibitory concentration (IC50) of fenvalerate to antibody hapten-HRP reaction was 193 μg/L with relative standard deviation (RSD, n=5) of 4.5% and the IC20 was 13.5 μg/L. The fenvalerate recoveries from peach determined by ELISA were 81%-89%, 85%-98% and 85%-106% with RSDs (n=5) of 5.1%, 5.6% and 7.7% at the spiked levels of 2 mg/kg, 0.2 mg/kg and 0.05 mg/kg, respectively. The minimum detection concentration of the method was 0.014mg/kg.