张余杰, 张亚, 董丁铭, 秦小萍, 孟霞. 坡柳皂苷对大菜粉蝶生长发育的抑制活性[J]. 农药学学报, 2014, 16(1): 102-105. DOI: 10.3969/j.issn.1008-7303.2014.01.17
    引用本文: 张余杰, 张亚, 董丁铭, 秦小萍, 孟霞. 坡柳皂苷对大菜粉蝶生长发育的抑制活性[J]. 农药学学报, 2014, 16(1): 102-105. DOI: 10.3969/j.issn.1008-7303.2014.01.17
    ZHANG Yujie, ZHANG Ya, DONG Dingming, QIN Xiaoping, MENG Xia. Inhibitory activity of Dodonaea viscosa saponin on the growth anddevelopment of Pieris brassicae[J]. Chinese Journal of Pesticide Science, 2014, 16(1): 102-105. DOI: 10.3969/j.issn.1008-7303.2014.01.17
    Citation: ZHANG Yujie, ZHANG Ya, DONG Dingming, QIN Xiaoping, MENG Xia. Inhibitory activity of Dodonaea viscosa saponin on the growth anddevelopment of Pieris brassicae[J]. Chinese Journal of Pesticide Science, 2014, 16(1): 102-105. DOI: 10.3969/j.issn.1008-7303.2014.01.17

    坡柳皂苷对大菜粉蝶生长发育的抑制活性

    Inhibitory activity of Dodonaea viscosa saponin on the growth anddevelopment of Pieris brassicae

    • 摘要: 采用浸渍叶碟饲喂法测定了坡柳皂苷对大菜粉蝶的生物活性。结果表明:用500、1 625、2 750、3 875和5 000 μg/mL的坡柳皂苷处理24 h后,对大菜粉蝶4龄幼虫生长发育的抑制率分别为14.47%、31.54%、49.70%、62.48%和79.94%,处理48 h后的抑制率分别为2.72%、39.26%、54.82%、70.53%和86.39%;坡柳皂苷对大菜粉蝶4龄幼虫具有显著的拒食活性,供试 5种浓度下处理24 h后,拒食率分别为30.21%、40.62%、54.33%、72.47%和84.84%,48 h后的拒食率为4.4% ~86.67%;5 000 μg/mL处理 14 d后,幼虫化蛹率为32%,显著低于对照组(72%);供试 5种浓度坡柳皂苷处理23 d后,蛹的羽化率分别为56%、44%、44%、36%和32%,均低于对照组(64%)。研究表明,坡柳皂苷对大菜粉蝶生长发育、取食及化蛹均有明显的抑制作用。

       

      Abstract: The biological activity of Dodonaea viscosa saponin to Pieris brassicae was determined by leaf dipping method. The results showed that the growth inhibition rates of 500, 1 625, 2 750, 3 875 and 5 000 μg/mL D.viscosa saponin treatments on the 4th instar larvae of P.brassicae were 14.47%, 31.54%, 49.70%, 62.48% and 79.94% respectively, at 24 h, and were 2.72%, 39.26%, 54.82%, 70.53% and 86.39% respectively, at 48 h. D.viscosa saponin had significantly antifeedant activity on the 4th instar larvae of P.brassicae, and the antifeedant rates of the 5 treatments of D.viscosa saponin mentioned above were 30.21%, 40.62%, 54.33%, 72.47% and 84.84% respectively, at 24 h, and were 4.4%-86.67% at 48 h. The P.brassicae larvae pupate rate of 5 000 μg/mL treatment was 32% after 14 d, which was significantly lower than that of the control (72%). And the P.brassicae emergence rate of the 5 treatments of D.viscosa saponin mentioned above were 56%, 44%, 44%, 36% and 32% respectively, after 23 d, which was lower than that of the control (64%). The results showed that D.viscosa saponin had significant inhibitory activity on growth, development, feeding and pupation of P.brassicae.

       

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