Abstract: One hundred and six single spore isolates of Fusarium graminearum were collected form 7 districts in Hubei province, and sensitivity of the isolates to tebuconazole and carbendazim was determined. The results showed that the sensitivity distribution of the 106 isolates was unimodal curves by W test (Shapiro-Wilk) of SAS statistical software; with EC50 values range (the effective concentration to inhibit mycelial growth by 50%) of 0.064 to 0.778 mg/L for tebuconazole and 0.090 to 0.858 mg/L for carbendazim. Statistical analysis showed that there was significant difference in EC50 values to both fungicides between populations from different districts. The isolates from Xiangyang showed significantly higher EC50 values than that of other populations (P=0.002). The results indicated that there was no resistant population of F. graminearum to tebuconazole and carbendazim detected in Hubei province, which suggested the two fungicides still have potential in controlling Fusarium head blight in the future.