江苏省水稻恶苗病菌对咪鲜胺和氰烯菌酯的敏感性
Sensitivity of Fusarium fujikuroi to prochloraz and JS399-19 in Jiangsu Province
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摘要: 对采自江苏省姜堰、靖江和常州3个地区的水稻恶苗病样品进行了病原菌的分离和鉴定,利用菌丝生长速率法测定了病原菌对咪鲜胺和氰烯菌酯的敏感性。结果表明:分离得到77株水稻恶苗病菌Fusarium fujikuroi;咪鲜胺对水稻恶苗病菌的EC50值在0.020~1.333 μg/mL之间,分离到的菌株对咪鲜胺均表现为中抗和高抗,其中中抗菌株18株,占23.38%,高抗菌株59株,占76.62%,说明咪鲜胺高抗菌株已经成为江苏省的恶苗病菌优势群体。氰烯菌酯(JS399-19)对水稻恶苗病菌的EC50值在0.012~2.040 μg/mL之间,菌株的敏感性频率近似正态分布,EC50均值为(0.684±0.265) μg/mL,建议将此值作为江苏省水稻恶苗病菌对氰烯菌酯的敏感性基线。Abstract: Samples of rice bakanae disease were collected from Jiangyan, Jingjiang, Changzhou in Jiangsu Province. Seventy seven isolates of Fusarium fujikuroi were identified, and their sensitivity to prochloraz and JS399-19 were investigated using mycelium growth inhibition method. The results exhibited that the EC50 values of prochloraz to F. fujikuroi isolates were from 0.020 to 1.333 μg/mL. Among the 77 F. fujikuroi isolates, 18 isolates exhibited medium level resistance to prochloraz, with a frequency of 23.38%;while 59 isolates showed high level resistance, with a frequency of 76.62%. The resistant population of F. fujikuroi to prochloraz was dominant in Jiangsu Province. The EC50 values of JS399-19 to F. fujikuroi isolates were from 0.0124 to 2.040 μg/mL, and the sensitivity frequency of F. fujikuro isolates to JS399-19 exhibited a Gaussian distribution, with mean EC50 value (0.684±0.265) μg/mL, which could be considered as sensitivity baseline of F. fujikuro to JS399-19 in Jiangsu Province.