Abstract: In order to develop environmental friendly and sustained released insect sex pheromone, in clusion interaction of (Z)-8-dodecylene-1-ol acetate (Z8-12:Ac) and β-cyclodextrin(β-CD) as major components of sex pheromone of Grapholitha molesta Busck was studied. The inclusion compound of Z8-12:Ac with β-CD was prepared by magnetic force agitation and characterized using Thin Layer Chromatogram (TLC) and Fourier-transform (IR). The optimal conditions for preparing this inclusion compound is: the concentration of β-CD is 0.044 g/mL, the ratio of Z8-12: Ac and β-CD is 0.20(g):4.0(g), the reaction was carried out at 60 ℃ for 0.5 h in water. verification test results matched well with the predicted results, which will provide reference for the future development of high performance sex pheromone products and application technology field.