Abstract: Mating interference hexyls butyrate microcapsules were prepared by phase separating, and ethyl cellulose was used as the shell material. The influence of the dosage of polyvinyl alcohol, the dosage of emulsifiers, the amounts of ethyl acetate and the quantities of release controlling agent n-dodecane was studied through orthogonal tests to obtain the optimum process. The morphology of microcapsules was scanned by biological microscope and the indexes of microcapsules, such as the drug loadings, the covering rate and the release rate, were determined. Finally, the field efficacy of the mating interference microcapsules was determined. The results showed that optimum preparation conditions were as follows: ethyl cellulose 3.0 g, hexyls butyrate 3.0 g, emulsifier 700# 1.5 g, polyvinyl alcohol 2.0 g, ethyl acetate 30 mL and n-dodecane 2.0 g. The drug loading and encapsulation rate of microcapsules prepared under the optimal conditions were 21.5% and 91.9%, respectively. The microcapsules had smooth appearance, uniform distribution of particle sizes and the average particle size was about 301.85 μ m. The releasing property was good and the release time was more than 35 days. The trapping of A.lucorum in control areas(666)was greater than the areas where placed with hexyls butyrate microcapsules(224). Hexyls butyrate microcapsules could interfere with the mating and then reduced the number of A.lucorum.