卢美光, 赵建周, 芮昌辉, 范贤林, 魏岑. 棉铃虫对Bt杀虫蛋白抗性测定方法的研究[J]. 农药学学报, 1999, 1(3): 61-66.
    引用本文: 卢美光, 赵建周, 芮昌辉, 范贤林, 魏岑. 棉铃虫对Bt杀虫蛋白抗性测定方法的研究[J]. 农药学学报, 1999, 1(3): 61-66.
    Lu Mei-guang, Zhao Jian-zhou, Rui Chang-hui, Fan Xian-lin, Wei Cen. DEVELOPMENT OF BIOASSAY METHODS FOR RESISTANCE MONITORING OF HELICOVERPA ARMIGERA HUBNER TO BACILLUS THURINGIENSIS INSECTICIDAL PROTEINS[J]. Chinese Journal of Pesticide Science, 1999, 1(3): 61-66.
    Citation: Lu Mei-guang, Zhao Jian-zhou, Rui Chang-hui, Fan Xian-lin, Wei Cen. DEVELOPMENT OF BIOASSAY METHODS FOR RESISTANCE MONITORING OF HELICOVERPA ARMIGERA HUBNER TO BACILLUS THURINGIENSIS INSECTICIDAL PROTEINS[J]. Chinese Journal of Pesticide Science, 1999, 1(3): 61-66.

    棉铃虫对Bt杀虫蛋白抗性测定方法的研究

    DEVELOPMENT OF BIOASSAY METHODS FOR RESISTANCE MONITORING OF HELICOVERPA ARMIGERA HUBNER TO BACILLUS THURINGIENSIS INSECTICIDAL PROTEINS

    • 摘要: 对饲料混合法 ,体重抑制率法 ,浸叶接虫法进行比较研究 ,结果表明 ,饲料混合法比较稳定 ,比较适宜作为抗性测定的方法。用饲料混合法测定比较不同龄期幼虫的测定结果 ,确定 2龄作供试幼虫为宜。比较不同处理天数下的生测结果 ,确定 Bt粉剂对 2龄幼虫的适宜处理时间为 5d,Cry1Ac为 14d。用改进稀饲料隔离饲养供试幼虫的方法后 ,得到理想的生测数据 ,生测时间延长到 14d,对照死亡率也能控制在 2 0 %以下。从我们 1998年对棉铃虫的抗性监测结果说明 ,尽管田间棉铃虫对 Bt ICP未达抗性水平 ,但对 Cry1Ac型蛋白敏感性已有降低 ,值得引起重视。

       

      Abstract: Three bioassay methods for monitoring the resistance of Helicoverpa armigera Hübner to Bacillus Thuringiensis Insecticidal Proteins were evaluated, ie dose response and growth inhibition assays with the test material incorporatied in diet and leaf dipping method. The results showed that the mortality from diet incorporation mothod using second instar larva is more suitable for resistance monitoring,and the best time for morality investigation was 5 days after Bt formulation treatment and 14 days after Cry1Ac treatment. The mortality of untreated larvae can be controlled below 20% by using improved artificial diet. Resistance Ratios were 4.6,3.9 and 3.8 folds to Cry1Ac and 1.1,1.4 and 1.6 folds to Bt formulation with Qiuxian,Jizhou and Gaomi populations in 1998 compared with a laboratory population using the diet incorporation mortality method.

       

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