闹羊花素-III对菜青虫神经系统的影响

Effects of Rhodojaponin-III on Nervous System of Larvae of Imported Cabbage Worm, Pieris rapae L

摘要: 以闹羊花素 - III处理 5龄菜青虫幼虫 ,试虫乙酰胆碱酯酶 (AChE)的活体活性和离体活性均与对照无明显差异 ,乙酰胆碱 (ACh)含量显著降低 ;对Na+ - K+ -ATP酶、Ca2+-Mg2+ - ATP酶比活力有可逆性激活作用 ,处理后24h比活力提高 ,尤以低剂量 1μg·虫-1处理的比活力显著提高 ;处理后 60h,以 1～ 3μg·虫-1处理的比活力恢复正常 ,以 5～ 10μg·虫-1处理的比活力显著低于对照。

Abstract: Effects of Rhodojaponin-III(Abbr.R-III) on nervous system of 5th instar larvae of imported cabbage worm, Pieris rapae L. under laboratory conditions were reported. Results showed that activities of acetylcholinesterase (AChE), both in vitro and in vivio were not significantly different from that of the control, while contents of acetylcholine (ACh) were remarkably decreased. R-III had reversible activated effects on Na+- K+ - ATPase and Ca 2+ -Mg2+ ATPase activities. R-III activated all ATPase activities when treated after 24h. A low dosage of 1 μg of R -IIIper larva significantly activated all ATPase activities over the control. It's observed that worms treated after 60h by 1～3μg R-IIIper larva recovered. While they were fed with 5～10μg R -III per larva, ATPase activities decreased to levels less than that of the control