核盘菌对菌核净的抗药性机制初探
Study on Resistance Mechanism of Sclerotinia sclerotiorum to Dimethachlon
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摘要: 经药剂筛选获得对菌核净不同表型的核盘菌(Sclerotinia sclerotiorum ) 抗药性突变体。与敏感亲本菌株相比, 抗药突变体MN 61 (MR ) 和MN 91 (HR) 在含1% 和8% 葡萄糖的PDA 上生长受到抑制,MN 113 (LR) 只对1% 葡萄糖敏感。通过测定抗药突变体MN 61 和野生敏感菌株PN 061 的电导率, 发现抗药突变体能在更短的时间里渗出更多的电解质。抗药突变体苯丙氨酸解氨酶活性比敏感亲本菌株高出1 倍以上, 当用不同浓度菌核净处理或饥饿处理时, 抗药突变体和敏感亲本菌株PAL 活性均上升, 但抗药突变体的酶活始终高于敏感亲本菌株。Abstract: The mutants in different resistence level of Sclerotinia sclerotiorum to dimethachlon were selected on PDA medium amended with the fungicide from 0.5 μg·ml -1 to 50.0 μg·ml -1 . The growth of mutants MN61(MR)and MN91(HR) was inhibited on the PDA containing 1% and 8% dextrose, and MN113(LR) was only sensitive to 1% dextrose compared with their parents incubated on the 2% dextrose medium. Dimethachlon caused more electrolyte leakage of the mutant cells than that of its parent isolate. The activity of PAL( L-phenylalanine ammonia lyase) of mutant MN61 was higher than that of sensitive isolate PN061 when they were incubated in the solution of nutrient deficieny or treated with 5 μg·ml -1 or 20 μg·ml -1 dimethachlon.