固相抗体直接竞争ELISA法测定小白菜和苹果中的甲萘威残留

The Residue of Carbaryl in Brassica chinensis and Apple Determined by Method of Antibody-Immobilized Direct Competitive ELISA

摘要: 采用固相抗体直接竞争ELISA法测定小白菜和苹果中的甲萘威残留量 ,并以高效液相色谱法进行验证。结果表明:ELISA法测定小白菜和苹果中甲萘威残留的检测限为0.4 ng/ kg;样本中添加 0.1mg/ kg和 10.0 mg/ kg甲萘威标样 ,以该法测定的回收率分别为80.9%～ 116 %和 86.7%～ 96.3% ,变异系数分别为 9.62 %～ 16.9%和 7.34%～ 11.4 % ;实际样本中甲萘威残留 ELISA测定的变异系数为 6.89%～ 10.3% ,符合残留分析的要求。方法简便快速 ,测定结果与高效液相色谱法测定结果基本一致。

Abstract: The method of antibody immobilized direct competitive ELISA was developed to monitor the residue of carbaryl in Brassica chinensis and apple. The results showed that the limit of detection was 0.4 ng/kg, the recoveries were 80.9%～116% and 86.7%～96.3%, the CV s were 9.62%～16.9% and 7.34%～11.4% at 0.100 mg/kg and 10.0 mg/kg spiking levels respectively. For the ELISA of carbaryl residue in real samples, the CV s were 6.89%～10.3%. The ELISA method is simple and rapid, the results from ELISA were well correspondent with those from HPLC.