Abstract: Toxicity of fungicides carbendazim, thiophanate-methyl+diethofencarb, difenoconazole, fludioxonil on Botrytis cinerea Pres.which causes gray mold of strawberry, was studied by using growing rate method. It was shown that the four fungicides have different effects in inhibition against Botrytis cinerea on strawberry and the effect is dependent on concentration of fungicides. The EC50 of fludioxonil carbendazim, thiophanate-methyl+diethofencarb, difenoconazole was 0.0472, 95.7193, 0.4854 and 1.3611 mg/mL, respectively. Among the four fungicides, the toxicity of fludioxonil is the highest. The field trial indicated that the control effect of 2.5% fludioconil against the pathogen were 83.7% and 67.7% at the concentration of 120 and 80 mL/667m2, 65% thiophanate-methyl+diethofencarb WP was 65.3% at the concentration of 100g/667 m2.