不同食料对甜菜夜蛾药剂敏感性及解毒酶活性的影响

Effect of Different Diets on the Susceptibility of Insecticides and the Activity of Detoxifying Enzymes in the Beet Armyworm, Spodoptera exigua

摘要: 甜菜夜蛾取食不同食料作物以后,对灭多威、辛硫磷和溴氰菊酯的敏感性均有不同程度的变化。解毒酶活性测定表明,不同食料对甜菜夜蛾3龄幼虫的多功能氧化酶(MFO)的脱甲基作用和羧酸酯酶(CarE)的活性有一定的诱导作用,和人工饲料相比,具有显著性差异。而羧酸酯酶动力学研究表明,几种食料作物对甜菜夜蛾羧酸酯酶底物亲和力(Km)的影响差异不显著。

Abstract: The susceptibility of the beet armyworm to methomyl,phoxim and deltamethrin, was various because of its feeding with different diets. Studies on detoxifying enzymes activities showed that the o-demethylation of MFO and activity of CarE in the 3 rd instar larvae of spodoptera exigua,could be induced by different diets, and indicated that the Km value of CarE in the larvae reared on different diets were not distinctly different from that of the control.