李少南, 谢显传, 谭亚军, 朱国念. 麦穗鱼脑AChE间接非竞争ELISA定量分析法的建立[J]. 农药学学报, 2003, 5(3): 71-75.
    引用本文: 李少南, 谢显传, 谭亚军, 朱国念. 麦穗鱼脑AChE间接非竞争ELISA定量分析法的建立[J]. 农药学学报, 2003, 5(3): 71-75.
    shu
    LI Shao-nan, XIE Xian-chuan, TAN Ya-jun, ZHU Guo-nian. Indirect and Non-competitive ELISA for Quantitative Analysis of the AChE from Topmouth Gudgeon[J]. Chinese Journal of Pesticide Science, 2003, 5(3): 71-75.
    Citation: LI Shao-nan, XIE Xian-chuan, TAN Ya-jun, ZHU Guo-nian. Indirect and Non-competitive ELISA for Quantitative Analysis of the AChE from Topmouth Gudgeon[J]. Chinese Journal of Pesticide Science, 2003, 5(3): 71-75.
    shu

    麦穗鱼脑AChE间接非竞争ELISA定量分析法的建立

    Indirect and Non-competitive ELISA for Quantitative Analysis of the AChE from Topmouth Gudgeon

      摘要
    • 摘要: 用PEG2000双水相萃取、 DEAE-Sephadex A-50和Sephadex G-200方法分离纯化麦穗鱼Pseudorasbora parva脑中的乙酰胆碱酯酶(AChE), 并制备了兔抗麦穗鱼脑AChE抗血清。用兔抗麦穗鱼脑AChE抗体连接抗原, 建立了定量分析麦穗鱼脑AChE的间接非竞争酶联免疫吸附法(Indirect and non-competitive ELISA), 此法操作简便、灵敏度高, 适用于定量检测。该方法的建立有利于AChE在环境科学和农药学研究领域的进一步研究和应用。

       

      Abstract: Acetylcholinesterase (AChE) was purified from the brain tissues of topmouth gudgeon (Pseudorasbora parva) by PEG 2000/phosphate salt with two phases extraction, DEAE-Sephadex A-50 and Sephadex G-200 chromatography, and rabbit anti-AChE antibody was made. Using rabbit anti-AChE antibody reacting with the brain AChE of topmouth gudgeon, indirect non-competitive ELISA was established to quantify the brain AChE of topmouth gudgeon. The method was easy to operate with high sensitive. The establishment of the method will be helpful for environmental pesticide science.

       

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