黄瓜霜霉病菌对嘧菌酯的敏感基线研究

Study on the Sensitive Baseline of Pseudoperonospora cubensis to the Strobilurin Fungicide Azoxystrobin

摘要: 从山东、河北、武汉等未使用过甲氧基丙烯酸酯类杀菌剂(strobilurins)的地区采集19个黄瓜霜霉病菌 Pseudoperonospora cubensis 样本,采用叶盘漂浮法室内测定新药剂嘧菌酯(azoxystrobin)对该病菌的毒力,其EC50值范围为11.72×10-5~9.340×10-3 μg/mL。在19个菌系中选择最为敏感的河北定兴菌系进行重复测定,将其EC50平均值2.317×10-4 μg/mL 确定为黄瓜霜霉病菌对嘧菌酯的敏感基线。

Abstract: Nineteen isolates of Pseudoperonospora cubensis were sampled from different regions in which the strobilurin fungicide had never been used. The toxicity of azoxystrobin to P. cubensis was assayed with leaf disk floating method. The EC50 values were in the range of 11.72×10-5~9.340×10-3 μg/mL, the sensitive baseline of P. cubensis to a strobilurin fungicide, azoxy-strobin, was determined as 2.317×10-4 μg/mL based on the mean EC50 value to the most sensitive isolate from Dingxing, Hebei.