Abstract: In order to separate and analyze fluroxypyr-meptyl enantiomers, cellulose-tris(3,5-dimethyl-phenylcarbamate) (CDMPC) was prepared and coated on aminopropylated spherical gel as a chiral stationary phase(CSP). Fluroxypyr-meptyl enantiomers were firstly and directly separated on this CSP by normal-HPLC, the effects of 2-propanol content in mobile phase and the flow rate on separation were investigated, and the interactive mechanism between CDMPC and enantiomers was discussed. When the mobile phase composed of hexane and 2-propanol(99∶1, V/V)and the flow rate was 0.5 mL/min,the separation factor(a)and the resolution factor(Rs) was 1.16 and 1.13,respectively. The results showed that good enantioseparation was achieved on CDMPC, accordingly a validated method can be developed to analyze fluroxypyr-meptyl enantiomers.