苍白杆菌B2对甲基对硫磷降解途径研究

Degradative Pathway of Parathion-Methyl by Ochrobacterum sp. B2

摘要: 采用薄层层析、紫外吸收光谱以及气谱-质谱联用的方法分析了苍白杆菌Ochrobacterum sp. B2降解甲基对硫磷的中间产物。结果表明,B2水解甲基对硫磷产生对硝基苯酚(PNP),PNP通过产生4-硝基邻苯二酚(4-nitrocatechol,4-NC)和1,2,4-苯三酚(1,2,4-benzenetriol) 的途径进一步代谢。苍白杆菌B2可以以PNP和4-NC为碳源生长,在菌的初始OD600值为0.02时,B2可在48 h内将50 mg/L的PNP完全降解, 在60 h内,将30 mg/L的4-NC完全降解。

Abstract: The parathion-methyl degradation by Ochrobacterum sp. B2 was analyzed by thin layer chromatography, UV spectroscopy and gas chromatography-mass spectrometry analysis. The results indicated that parathion-methyl was hydrolyzed to p-nitrophenol, and this metabolite was further degraded to 4-nitrocatechol and 1,2,4-benzenetriol. B2 could grow on a medium with p-nitrophenol or 4-nitrocatechol as a sole carbon source. 50 mg/L p-nitrophenol was degraded completely in 48 h and 30 mg/L 4-nitrocatechol disappeared almost in 60 h when the initial OD600 of B2 cells was 0.02.