聚乙二醇8000对棉铃虫微粒体蛋白沉淀作用的研究

Precipitation of Micorsomal Proteins in Helicoverpa armigera by PEG8000

摘要: 为建立棉铃虫Helicoverpa armigera微粒体P450的分离纯化方案,比较了不同浓度下聚乙二醇8000(PEG8000)对棉铃虫幼虫中肠和脂肪体微粒体蛋白的沉淀作用。结果表明,终浓度为8.0×104 mg/L的PEG8000可以使中肠和脂肪体微粒体蛋白的78%沉淀下来,其中包含的细胞色素P450分别占中肠和脂肪体P450总量的28%和34%。SDS-PAGE显示,中肠微粒体经8.0×104 mg/L的PEG8000沉淀后,被沉淀蛋白的分子质量主要集中在14.1 ~ 40 kD和66 ~97 kD范围内。

Abstract: In order to develop a protocol for purifying cytochrome P450 in the cotton bollworm, Helicoverpa armigera, the precipitation results of PEG8000 at different concentration to microsomal proteins of cotton bollworm were compared. The results showed that at the concentration of 8.0×104 mg/L, 78% microsomal proteins including 28% total P450 of midgut microsome or 34% total P450 of fatbody microsome were precipitated. SDS-PAGE showed that most of the precipitated microsomal proteins from midgut were those which molecular weight was among the range of 14.1 ~40 kD and 66 ~97 kD.