Abstract: With the extraction efficiency of rotenone and total substance obtained as estimated index, the technology conditions of extracting rotenone from the leaves of Tephrosia vogelii were investigated by SFE-HPLC method. The rational conditions were sample size of 30 mesh, time of static extraction before dynamic extracting 20 min, temperature 45℃, pressure 27.58 MPa, flux of fluid 30 mL/g, and add 3 mL methanol as modifier into extraction tube. The technique of SFE under this condition was compared with chloroform-soaked. The results showed that 98.0% rotenone of T. vogelii can be extracted with SFE. The investigated SFE conditions is suitable to the extraction, separation and analysis of rotenone from botanical material. Moreover, SFE technique is simple and convenient, and has the merits of high efficiency, accuracy, innocuity, economization, and selectivity, and so on.