丛林晔, 丁秀英, 王毓, 何永明, 陈淑君, 张军. 不同抗逆诱导剂对水稻细胞PBZ 1 基因表达的影响[J]. 农药学学报, 2005, 7(3).
    引用本文: 丛林晔, 丁秀英, 王毓, 何永明, 陈淑君, 张军. 不同抗逆诱导剂对水稻细胞PBZ 1 基因表达的影响[J]. 农药学学报, 2005, 7(3).
    CONG Lin-ye, DING Xiu-ying, WANG Yu, HE Yong-ming, CHEN Shu-jun, ZHANG Jun. Effect on Expression of PBZ 1 in Rice Suspension Cell by Different Stress-resistance Inducers[J]. Chinese Journal of Pesticide Science, 2005, 7(3).
    Citation: CONG Lin-ye, DING Xiu-ying, WANG Yu, HE Yong-ming, CHEN Shu-jun, ZHANG Jun. Effect on Expression of PBZ 1 in Rice Suspension Cell by Different Stress-resistance Inducers[J]. Chinese Journal of Pesticide Science, 2005, 7(3).

    不同抗逆诱导剂对水稻细胞PBZ 1 基因表达的影响

    Effect on Expression of PBZ 1 in Rice Suspension Cell by Different Stress-resistance Inducers

    • 摘要: 以水稻品种中花8号的悬浮细胞为试材,分别研究了真菌激发子和盐激发子对用稻瘟灵(IPT)和脱落酸(ABA)预处理24 h的水稻悬浮细胞中PBZ 1 基因表达的影响。结果发现:单独用IPT、ABA处理可以诱导细胞中PBZ 1 基因表达;只用真菌激发子和盐激发子处理的细胞中也可以检测到PBZ 1 基因的表达,但两种激发子对PBZ 1 基因表达的影响不同,在真菌激发子处理后的0~3 h之间PBZ 1 基因表达逐渐增强,而在盐激发子处理后的0~3 h之间则呈现逐渐减弱的趋势,到第3 h就检测不到了;用IPT预处理24 h后,再分别用真菌激发子和盐激发子诱导,水稻细胞中PBZ 1 基因表达强度都显著高于单独用IPT处理的细胞,而且在处理后的第0.5~3 h期间一直保持较强的表达;ABA+盐激发子处理使细胞中PBZ 1 基因表达推迟且短暂,只在处理后第1~2 h之间可检测到,ABA+真菌激发子处理使细胞中PBZ 1 基因在0.5~3 h期间一直保持较强的表达,明显强于单独用ABA处理的细胞。可见,IPT和ABA都可以诱导抗病基因PBZ 1 的表达,PBZ 1 基因也可由盐激发子诱导表达,说明PBZ 1 基因对除病害以外的非生物逆境也有积极的作用。

       

      Abstract: The effect on expression of PBZ 1 in rice suspension cell (Zhonghua 8)induced by the elicitors of fungal and salt pretreated with isoprothiolane (IPT) and abscisic acid (ABA) for 24 h were studied. The results showed that IPT or ABA or the two elicitors treatment could separately induce the expression of PBZ 1 in the suspension cell and without the expression of PBZ 1 in the control cells was observed treated with only the fungal and salt elicitors.The expression of PBZ 1 enhanced gradually from zero to three hours after treated with fungal elicitor and weakened gradually to zero within the same interval treated by salt elicitor. The expression intensity of PBZ 1 in rice cell treated only with IPT was obviously higher than that of induced by elicitor of fungal or salt pretreated by IPT for 24 h, and a continuance of high expression of PBZ 1 was observed from 0.5 to 3 h after treatment as well as by fungal elicitor after ABA treatment. It is obvious that IPT, ABA and fungal could induce the expression of PBZ 1 . The PBZ1 gene had active effects on the abiotic stresses with the exception of diseases.

       

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