牛洪涛, 宗建平, 王海迎, 魏书娟, 朱秀凤, 罗万春. 小菜蛾对丁烯氟虫腈的抗性选育及生物适合度[J]. 农药学学报, 2007, 9(3): 245-250.
    引用本文: 牛洪涛, 宗建平, 王海迎, 魏书娟, 朱秀凤, 罗万春. 小菜蛾对丁烯氟虫腈的抗性选育及生物适合度[J]. 农药学学报, 2007, 9(3): 245-250.
    NIU Hong-tao, ZONG Jian-ping, WANG Hai-ying, WEI Shu-juan, ZHU Xiu-feng, LUO Wan-chun. Resistance Selection of Plutella xylostella to Butene-fipronil and its Growth Fitness[J]. Chinese Journal of Pesticide Science, 2007, 9(3): 245-250.
    Citation: NIU Hong-tao, ZONG Jian-ping, WANG Hai-ying, WEI Shu-juan, ZHU Xiu-feng, LUO Wan-chun. Resistance Selection of Plutella xylostella to Butene-fipronil and its Growth Fitness[J]. Chinese Journal of Pesticide Science, 2007, 9(3): 245-250.

    小菜蛾对丁烯氟虫腈的抗性选育及生物适合度

    Resistance Selection of Plutella xylostella to Butene-fipronil and its Growth Fitness

    • 摘要: 用丁烯氟虫腈对小菜蛾敏感品系在室内经7代次药剂汰选,获得了抗性品系(R),与敏感品系(S)比较其抗性指数为77.58倍。抗性汰选前后,采用点滴法测定的结果表明,小菜蛾对阿维菌素、毒死蜱、灭多威、杀虫单和高效氯氰菊酯的敏感性并无明显变化,其抗性指数为0.80~1.36,均不存在交互抗性。通过构建种群生命表,观察比较了小菜蛾抗丁烯氟虫腈品系和敏感品系的一系列生长发育和繁殖特征。结果表明,抗丁烯氟虫腈品系较敏感品系幼虫期和蛹历期延长,低龄幼虫龄期转换时的虫口死亡率增加,蛹重减轻,化蛹率和羽化率降低;在繁殖上表现为产卵量增加,而孵化率和雌雄比降低,雌成虫的数量、寿命减少。抗丁烯氟虫腈种群相对于敏感种群的相对适合度为0.84,抗性种群在繁殖能力上存在明显的生存劣势。

       

      Abstract: After selection for resistance to butene-fipronil for 7 generations,the resistance ratio of the fourth instar larvae of diamondback moth,Plutella xylotella(L.)to butene-fipronil increased 77.58-folds.The susceptibility of butene-fipronil-resistant larvae to different insecticides were tested with topical application method.The results indicated that the butene-fipronil-resistant larvae showed no cross resisitance to abamectin,chlorpyrifos,methomyl,monosultap or beta-cypermethrin.The resistance ratio estimated to be 0.80~1.36-folds.The effects of butene-fipronil resistance on fitness of the diamondback moth were evaluated in terms of developmental and reproductive characteristics by constructing life table of resistance strain and susceptible strain.The results indicated that the resisitance strain had a fitness value of 0.84 relative to the susceptible one.Comparing with the susceptible strain,the resisitance strain possessed developmental disadvantages including prolonged larvae and pupa duration,decreased pupa body weight, ower pupation and emergence rate,as well as reproductive disadvantages including increased egg laying amount,lowered hatch ability and sex ratio (♀:♂),reduced life span and number of female adults.

       

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