马拉硫磷对映体对人成神经细胞瘤细胞生长影响的选择性差异
Effect of Enantiomerselectivity of Malathion on the Growth of Human Neuroblast Cells
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摘要: 首次报道了马拉硫磷及其对映体对神经细胞生长的影响及其对映体对神经细胞毒性的选择性差异。以人成神经细胞瘤(SH-SY5Y)细胞为体外研究模型,利用高效液相色谱法对马拉硫磷进行手性拆分,分别研究其外消旋体(rac-)、R- 和S-体对神经细胞生长的影响。用10-7~10-4 mol/L 的马拉硫磷及其对映体分别处理SH-SY5Y细胞,通过噻唑兰(MMT)法分析细胞的生长率,同时用显微镜观察细胞的生长状态以评价化合物的毒性。结果表明:SH-SY5Y细胞生长受到马拉硫磷的抑制,且对时间、剂量呈现出一定的依赖性。10-4 mol/L浓度下其抑制作用最为显著,在该浓度下处理48 h马拉硫磷R-体对细胞生长的相对抑制率为75%左右,比S-体的作用强近2倍。显微观察结果显示,处理后的SH-SY5Y细胞形态发生了一定的变化,出现细胞变形、增大等现象。研究结果表明,马拉硫磷对SH-SY5Y神经细胞生长具有一定的抑制作用,且其对神经细胞的毒性存在着明显的对映体选择性。Abstract: The effect of human neuroblast cells (SH-SY5Y) growth by malathion and the stereoselectivity of malathion were analyzed for the first time.After the enantiomeric separation of mamathion by HPLC, the SH-SY5Y cells were treated by 10-7~10-4 mol/L malathion,R-and S-malathion enantiomers respectively. The cell growth was measured by MTT, and cell morphology was observed by microscope. The results showed that SH-SY5Y cell growth was obviously inhibited by rac-,R- and S-malathion with dose and time fashion. 10-4 mol/L Malathion and its enantiomers displayed the most notable inhibition, and at that concentration the R-malathion inhibited the cell growth 2 times stronger than S-malathion after the treatment of 48 h, the relative inhibition ratio was about 75%. Meanwhile the results showed by microscope that cell morphology has been certain changed, such as deformation and size increasing.
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