胡林峰, 冯俊涛, 张兴, 张应烙. 孜然种子中杀菌活性成分分离及结构鉴定[J]. 农药学学报, 2007, 9(4): 330-334.
    引用本文: 胡林峰, 冯俊涛, 张兴, 张应烙. 孜然种子中杀菌活性成分分离及结构鉴定[J]. 农药学学报, 2007, 9(4): 330-334.
    HU Lin-feng, FENG Jun-tao, ZHANG Xing, ZHANG Ying-luo. Isolation and Structure Detection of Fungicidal Components from Cuminum cyminum Seed[J]. Chinese Journal of Pesticide Science, 2007, 9(4): 330-334.
    Citation: HU Lin-feng, FENG Jun-tao, ZHANG Xing, ZHANG Ying-luo. Isolation and Structure Detection of Fungicidal Components from Cuminum cyminum Seed[J]. Chinese Journal of Pesticide Science, 2007, 9(4): 330-334.

    孜然种子中杀菌活性成分分离及结构鉴定

    Isolation and Structure Detection of Fungicidal Components from Cuminum cyminum Seed

    • 摘要: 以小麦白粉病菌Blumer graminis、黄瓜霜霉病菌Pseudoperonispora cubensis、杨树溃疡病菌Dothiorella gregaria、棉花枯萎病菌Fusarium oxysporum f.sp.vasinfectum、白菜黑斑病菌Alternaria oleracea和稻瘟病菌Pyricularia grisea 6种病原菌为指示菌种,对孜然种子中的杀菌活性成分进行了跟踪分离及活性测定。采用柱层析分离技术,从孜然种子乙醇浸膏石油醚萃取液中分离得到两个具有杀菌活性的化合物,其结构经质谱、核磁共振氢谱及碳谱等分析确定其分别为枯茗酸(对异丙基苯甲酸,p-isopropyl benzoic acid)和枯茗醛(对异丙基苯甲醛,p-isopropyl benzaldehyde)。采用菌丝生长速率法测定了两化合物对油菜菌核病菌Sclerotinia sclerotiorum和辣椒疫霉Phytophthora capsici的毒力,其中枯茗醛对两种病原菌的EC50值分别为2.093和 15.40 mg/L,枯茗酸的EC50值分别为7.298和19.66 mg/L。

       

      Abstract: Six plant pathogens (Pyricularia grisea,Sclerotinia sclerotiorum,Phytophthora capsici,etc.) were used to screen fungicidal components from Cuminum cyminum L.seed.Cumin seeds were distilled by alcohol,and then extracted by petroleum ether,chloroform,ethyl ester,and n-butanol in sequence.Two compounds were isolated from petroleum ether extract by column chromatography.Their structures were identified as p-isopropyl benzoic acid and p-isopropyl benzaldehyde by MS and NMR.The inhibitive rate against the mycelial growth of S.sclerotiorum,P.capsici were tested.The EC50 of p-isopropyl benzaldehyde against S.sclerotiorum and P.capsici were 2.093 and 15.40 mg/L,respectively.The EC50 of p-isopropyl benzoic acid against S.sclerotiorum and P.capsici were 7.298 and 19.66 mg/L,respectively.

       

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