Abstract: The content of triptolide and the total alkaloids in the root bark,leaves,the cultures and the nutrient solution were determined based on the determination of triptolide by HPLC and UV-spectroscopy method for total alkaloids in the same extract.Samples were extracted with ethyl acetate and cleaned up on an aluminum column,followed by dissolution in 45% methanol solution.The results showed that the linear relationship of the triptolide concentrations and peak areas was in the range of 1~100 μg/mL (R2=0.999 9).The linear relationship of the total alkaloids concentrations and absorbance was good in range of 5~100 μg/mL (R2=0.999 8).In the same extract the average recovery of triptolide was 100.5% (RSD=0.63%)and the average recovery of total alkaloids was 99.8% (RSD=1.22%).It's found that the content of triptolide in the root callus was about 1.25 times as that in the root bark.The yield of triptolide was 56% and that of the total alkaloids was 65% after filtering adventitious roots in the culture medium.The triptolide yield of 1 g per adventitious root was about 10 times that of root bark.The total alkaloids yield of 1 g per adventitious root was about 2 times that of root bark.