Abstract: Repeated and unreasonable field application of herbicides have resulted in the evolution of resistant weed species.AHAS is the target site of AHAS-inhibiting herbicides.By 2009,103 weed biotypes have showed resistance to AHAS-inhibiting herbicides,accounting for nearly 1/3 of total weed biotypes resistant to 19 chemical groups.The relationship between AHAS gene mutation sites/types and the level of herbicide resistance,AHAS gene mutations and AHAS activity,AHAS gene number and herbicide resistance,and the 3D structure of AHAS before and after binding with AHAS-inhibiting herbicides were reviewed.ECOTILLING and dCAPS,two new techniques for rapidly diagnosing herbicide resistance by detecting SNP were brought forward,and potential management strategy for preventing and delaying the herbicide resistance evolution in weeds was also discussed.