Abstract: Bioremediation of Klebsiella sp. 2N3 strain in chlorimuron-ethyl contamined soil was examined under laboratory conditions. When 2N3 was inoculated into the soil at the density of 1×106 mycelium/g dry soil,chlorimuron-ethyl ( 20 mg/kg dry soil ) in soil was degraded by 84.6% after incubated for 30 days, with blank control of 13.4% degradation rate.When chlorimuron-ethyl was increased by 100 mg /kg in soil, chlorimuron-ethyl degradation rate was below 31.1%. With addition of chlorimuron-ethyl of 20 mg/kg in soil, and amount of strain 2N3 in 1×106 mycelium /g dry soil, emergence rates of wheat, corn and cucumber were 85%,82% and 79%,respectively. Plant heights were observed to increase in comparison with control plant.It's indicated that phyto-toxicity effect of chlorimuron-ethyl could be retarded by strain 2N3. The current study showed that the hand-actuated inoculation of 2N3's strain could effectively increase degradation rate of chlorimuron-ethyl in contaminated soil .