枸骨内生菌No.2的鉴定及其对黄瓜立枯病的生防作用

Identification of an endophytic isolate from Llex cornuta andits antagonism against Rhizoctonia solani

摘要: 从枸骨植物中分离到一株对立枯丝核菌Rhizoctonia solani具有抑制作用的内生真菌(编号为No.2),借助Biolog微生物自动分析仪对该真菌进行了分类鉴定,根据菌株的生理生化和形态特征,初步确定该菌株为哈茨木霉菌Trichoderma harzianum Rifai。双重培养试验表明,该哈茨木霉菌对黄瓜立枯病菌存在竞争和寄生作用;盆栽试验测定结果表明,喷施哈茨木霉菌胞外代谢产物木霉素的1 000倍液7 d后,其对黄瓜立枯病的保护和治疗效果分别为88.89%和95.83%。施药后各处理对黄瓜的株形、叶色均未产生药害症状,表明其安全性较好。

Abstract: An endophytic isolate (No.2),which has inhibiton on Rhizoctonia solani,was isolated from Ilex cornuta Lindl.et.Paxt.It was identified as Trichoderma harzianum Rifai by morphological and physiological & biochemical characteristics via Biolog Microbial Identification System.The test of dual culture indicated that there were competitive and mycoparasitic behavior by T.harzianum on R.solani.Trichodermin isolated from the broth of T.harzianum showed strong biological activity against R.solani the protective effects and therapeutic effects were 88.89% and 95.83%,respectively,after treatment for 7 days.There was no blight on cucumber seedlings observed after treatment of trichodermin.The results indicated that trichodermin was safe to cucumber plants.