抑制番茄早疫病菌活性化合物的 高通量筛选方法研究

抑制番茄早疫病菌活性化合物的高通量筛选方法研究

摘要: 以OD 630 值作为评价指标,在高通量筛选设备上建立了番茄早疫病菌Alternaria solania的高通量定量筛选方法,并对嘧菌酯、百菌清、 NFDA1 醚唑、霜脲氰、甲霜灵、代森锰锌、福美双和咪鲜胺8种常用杀菌剂对番茄早疫病菌孢子萌发和菌丝生长的抑制效果进行了评价,结果与孢子萌发法和目测筛选法所得结论基本一致。

Abstract: With OD630 value as an evaluation index,high-throughput screening method for discovering agrochemicals effective on tomato early blight pathogen was established by a micro plate spectrophotometer.The effect of eight widely-used fungicides(including azoxystrobin,chlorthalonil,difenoconazole,cymoxanil,metalaxyl,mancozeb,thiram and prochloraz) against Alternaria solani spore germination and mycelial growth was tested.The conclusion from high-throughput screening method was almost consistent with that from conventional spore germination method or visual observation.