Abstract: A reversed phase high-performance liquid chromatography(HPLC) method has been developed for carbendazim residue in Radix Scrophulariae,Radix Scutellariae and Flos Chrysanthemi.Carbendazim was extracted with acetone,cleaned up by column chromatography(florisil-neutral alumina=7∶ 4,m/m) and followed by liquid-liquid partition.HPLC conditions were as follows:Diamonsil C18(2) column with temperature at 30℃,methanol-water(60∶ 40,V/V) as mobile phase with flow rate of 0.8 mL/min and the detection wave length at 281 nm.The fortified recoveries and relative standard deviation(RSD) were from 69.08% to 88.31% and 3.35% to 4.67% in Radix Scrophulariae,from 71.39% to 88.40% and 3.73% to 8.62% in Radix Scutellariae,from 73.33% to 97.31% and 2.83% to 9.44% in Flos Chrysanthemi.The standard curve of carbendazim was linear in the range of 0.05-10 mg/L(r=0.999 7),the limit of detection(S/N=3) was 0.3,0.5 and 0.3 ng,the limit of quantification was 0.01,0.05 and 0.01 mg/kg,respectively.The analytical method is easier,faster,satisfied with the analysis of carbendazim residue in three kinds of Chinese herbal medicines and has better purification effect.