李拓, 张友军, 王少丽, 吴青君, 徐宝云, 杨中侠, 王沫. Cry1Ac敏感和抗性小菜蛾bre-3和bre-5 基因mRNA的表达差异研究[J]. 农药学学报, 2011, 13(1): 13-20.
    引用本文: 李拓, 张友军, 王少丽, 吴青君, 徐宝云, 杨中侠, 王沫. Cry1Ac敏感和抗性小菜蛾bre-3和bre-5 基因mRNA的表达差异研究[J]. 农药学学报, 2011, 13(1): 13-20.
    shu
    LI TUO, ZHANG You-jun, WANG Shao-li, WU Qing-jun, XU Bao-yun, YANG Zhong-xia, WANG Mo. Research in expression differences of bre-3 and bre-5 mRNA in Plutella xylostella susceptible and resistant to Cry1Ac[J]. Chinese Journal of Pesticide Science, 2011, 13(1): 13-20.
    Citation: LI TUO, ZHANG You-jun, WANG Shao-li, WU Qing-jun, XU Bao-yun, YANG Zhong-xia, WANG Mo. Research in expression differences of bre-3 and bre-5 mRNA in Plutella xylostella susceptible and resistant to Cry1Ac[J]. Chinese Journal of Pesticide Science, 2011, 13(1): 13-20.
    shu

    Cry1Ac敏感和抗性小菜蛾bre-3和bre-5 基因mRNA的表达差异研究

    Research in expression differences of bre-3 and bre-5 mRNA in Plutella xylostella susceptible and resistant to Cry1Ac

      摘要
    • 摘要: 在室内选用Cry1Ac对小菜蛾Plutella xylostella 3龄幼虫进行抗性选育,获得相对抗性为356倍的抗性种群DBM1Ac-R品系。应用实时定量基因扩增荧光检测(real time quantitative PCR,real-time qPCR)技术检测敏感小菜蛾DBM1Ac-S和抗性小菜蛾DBM1Ac-R品系鞘糖脂(Glycosphingolipids,GSLs)合成酶基因bre-3和bre-5 mRNA在2龄、3龄、4龄和老熟幼虫、蛹及4龄中肠的表达情况。结果显示:bre-3和bre-5在两个品系的5个时期和4龄中肠均有表达,其中DBM1Ac-S品系的bre-3 mRNA在3龄、4龄和老熟幼虫的相对表达量显著高于DBM1Ac-R品系(PPBacillus thuringiensis,Bt)杀虫晶体蛋白受体之一GSLs的进一步研究提供了一定的基础。

       

      Abstract: DBM1Ac-R strain of Plutella xylostella was selected with Cry1Ac in laboratory and showed 356-fold resistance to Cry1Ac compared with a susceptible strain (DBM1Ac-S).The relative expression of synthetase gene of Glycosphingolipid(GSLs) bre-3 and bre-5 mRNA of DBM1Ac-S and DBM1Ac-R strains were determined using real-time qPCR,from 2nd-instar to mature larvae,pupa and midgut of 4th-instar.The results showed that both bre-3 and bre-5 mRNA were found to express in different development stages and midgut;The relative expression of bre-3 mRNA in DBM1Ac-S strain was significant higher than DBM1Ac-R strain,exhibiting 2.36,5.54 and 2.68 times in 3th-instar,4th-instar and mature larvae respectively;The relative expression of bre-5 mRNA in DBM1Ac-S strain were 1.19,3.13,1.78,1.75 and 1.65 times respectively compared with DBM1Ac-R strain from 2th-instar to mature larvae.The results suggested that there may be a correlation between the lower relative expression of bre-3 and bre-5 mRNA in DBM1Ac-R from 3th to mature larvae and the long-term Cry1Ac toxin selection to DBM1Ac-R strain.In the same stage of DBM1Ac-S and DBM1Ac-R strains,the mRNA level expression of bre-5 was higher than bre-3,showing obvious advantage of gene expression.These findings will provide important basis for further study on GSLs,one of the receptors of Bacillus thuringiensis crystal proteins.

       

      • HTML全文
      • 参考文献(30)
      • 相关文章
      • 施引文献
    • 资源附件(0)

    /

    返回文章
    返回