三唑醇对映体的反相高效液相色谱拆分热力学研究

王新全; 王雪松; 王祥云; 徐浩; 章虎; 李振

三唑醇对映体的反相高效液相色谱拆分热力学研究

Thermodynamic property of the enantiomeric separation for triadimenol by reverse-phase high-performance liquid chromatography

摘要

摘要: 在高效液相色谱反相条件下,利用涂敷型纤维素-三(3,5-二甲基苯基氨基甲酸酯)手性固定相(CDMPC-CSP)对三唑醇4个对映异构体进行了直接手性拆分研究。考察了不同流动相组成和柱温对三唑醇对映体的色谱保留及分离的影响。同时,利用热力学方法对三唑醇对映体与固定相之间的色谱保留和分离的热力学机理进行了探讨。结果表明:在以甲醇-水(70∶ 30,体积比)为流动相、流速0.8 mL/min、柱温15 ℃,或在以乙腈-水(30∶ 70,体积比)为流动相、流速1.0 mL/min、柱温45 ℃的条件下,三唑醇对映体可以得到较好分离。

Abstract: Enantiomeric separation of triadimenol was performed by reverse-phase high-performance liquid chromatography (HPLC) on the coating-type cellulose tris (3,5-dimethylphenylcarbamate) chiral stationary phase (CDMPC-CSP).The effects of different mobile phase compositions and temperature of column on the retention and resolution in the chiral separation of triadimenol were investigated.Moreover,the enantiomeric separation mechanism of triadimenol enantiomer on the CDMPC-CSP was analyzed by thermodynamical parameters.The results showed that the ideal resolution of triadimenol on CDMPC-CSP could be achieved with the mobile phase of methanol-water (70∶ 30,V/V) at a flow rate of 0.8 mL/min and at 15 ℃,or could be achieved with the mobile phase of acetonitrile-water (30∶ 70,V/V) at a flow rate of 1.0 mL/min and at 45 ℃.

HTML全文

参考文献(14)

施引文献

资源附件(0)